Anti-SHARP2/DEC1抗体(ab70723)
Key features and details
- Rabbit polyclonal to SHARP2/DEC1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SHARP2/DEC1抗体
参阅全部 SHARP2/DEC1 一抗 -
描述
兔多克隆抗体to SHARP2/DEC1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human SHARP2/DEC1 (C terminal).
Database link: NP_003661.1 -
阳性对照
- Whole cell lysates from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70723 was affinity purified using an epitope specific to SHARP2/DEC1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70723于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
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| IP |
Use at 1-4 µg/mg of lysate.
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| 说明 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). |
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IP
Use at 1-4 µg/mg of lysate. |
靶标
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功能
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1
ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway. -
组织特异性
Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach. -
序列相似性
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 Orange domain. -
翻译后修饰
Ubiquitinated; which may lead to proteasomal degradation.
Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity. -
细胞定位
Cytoplasm. Nucleus. Predominantly localized in the nucleus (PubMed:11278694). - Information by UniProt
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数据库链接
- Entrez Gene: 8553 Human
- Omim: 604256 Human
- SwissProt: O14503 Human
- SwissProt: Q5RA17 Orangutan
- Unigene: 744856 Human
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别名
- BHE40_HUMAN antibody
- bHLHB2 antibody
- bHLHe40 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARP2/DEC1 antibody (ab70723)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2/DEC1 with ab70723 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-SHARP2/DEC1 antibody (ab70723)Anti-SHARP2/DEC1 antibody (ab70723) at 0.1 µg/ml + Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-SHARP2/DEC1 antibody (ab70723)Detection of Human SHARP2/DEC1 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2/DEC1 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab70723 被引用在 5 文献中.
- Zhou L et al. A bioinformatics analysis and an experimental validation of the hypoxia-related prognostic model. J Gastrointest Oncol 14:1504-1524 (2023). PubMed: 37435230
- Hu X et al. Overexpression of DEC1 in the epithelium of OSF promotes mesenchymal transition via activating FAK/Akt signal axis. J Oral Pathol Med 51:780-790 (2022). PubMed: 36053880
- Lv W et al. Differentiated embryonic chondrocyte-expressed gene 1 promotes temozolomide resistance by modulating the SP1-MGMT axis in glioblastoma. Am J Transl Res 13:2331-2349 (2021). PubMed: 34017393
- Mao T et al. DEC1: a potential biomarker of malignant transformation in oral leukoplakia. Braz Oral Res 34:e052 (2020). PubMed: 32578762
- Hwang JW et al. iPSC-Derived Embryoid Bodies as Models of c-Met-Mutated Hereditary Papillary Renal Cell Carcinoma. Int J Mol Sci 20:N/A (2019). PubMed: 31575031