重组Anti-SH3GL1/Endophilin A2抗体[K51/1] (ab317835)
![Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)](https://www.abcam.cn/ps/products/317/ab317835/Images/ab317835-546805-anti-sh3gl1endophilin-a2-antibody-k511-western-blot-rat-brain-tissue-lysate20-ug.jpg "Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [K51--1] to SH3GL1/Endophilin A2
- Suitable for: IP, WB, IHC-P
- Reacts with: Rat
Related conjugates and formulations
概述
-
产品名称
Anti-SH3GL1/Endophilin A2抗体[K51/1]
参阅全部 SH3GL1/Endophilin A2 一抗 -
描述
小鼠单克隆抗体[K51--1] to SH3GL1/Endophilin A2 -
宿主
Mouse -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Rat
不与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Rat brain, Rat spleen, PC-12, C6, L6, AR42J and H9c2 lysates. IHC-P: Rat cerebrum and Rat cardiac muscle tissues. IP: Rat brain and Rat testis cells.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
K51--1 -
同种型
IgG1
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317835于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000. Predicted molecular weight: 41 kDa.
|
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IP
1/30. |
|
WB
1/1000. Predicted molecular weight: 41 kDa. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
-
功能
Implicated in endocytosis. May recruit other proteins to membranes with high curvature. -
组织特异性
Ubiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus. -
疾病相关
In some cases of acute leukemia, a translocation results in the formation of a KMT2A/MLL1-EEN fusion gene. -
序列相似性
Belongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain. -
结构域
An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. -
细胞定位
Cytoplasm. Early endosome membrane. Cell projection, podosome. Associated with postsynaptic endosomes in hippocampal neurons. - Information by UniProt
-
数据库链接
-
别名
- EEN antibody
- EEN fusion partner of MLL antibody
- Endophilin-2 antibody
see all
图片
-
Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)All lanes : Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: heart and kidney (PMID: 11518713).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)](https://www.abcam.cn/ps/products/317/ab317835/Images/ab317835-546806-anti-sh3gl1endophilin-a2-antibody-k511-western-blot-pc-12-rat-adrenal-gland-pheochromocytoma-cell-whole-cell-lysate20-ug.jpg)
Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)All lanes : Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 3 : L6 (rat skeletal muscle myoblast) whole cell lysate
Lane 4 : AR42J (rat pancreatic tumor epithelial cell) whole cell lysate
Lane 5 : H9c2(2-1) (rat cardiomyoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)](https://www.abcam.cn/ps/products/317/ab317835/Images/ab317835-3-anti-sh3gl1endophilin-a2-antibody-k511-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining on rat cerebrum.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)](https://www.abcam.cn/ps/products/317/ab317835/Images/ab317835-4-anti-sh3gl1endophilin-a2-antibody-k511-immunohistochemistry-rat-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: weak staining on rat cardiac muscle.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)](https://www.abcam.cn/ps/products/317/ab317835/Images/ab317835-5-anti-sh3gl1endophilin-a2-antibody-k511-immunoprecipitation-rat-brain-tissue-lysate.jpg)
Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: ab317835 IP in Rat brain tissue lysate
Lane 3: Mouse monoclonal IgG (ab18443) instead of ab317835 in rat testis tissue lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
-
![Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)](https://www.abcam.cn/ps/products/317/ab317835/Images/ab317835-6-anti-sh3gl1endophilin-a2-antibody-k511-immunoprecipitation-rat-testis-tissue-lysate.jpg)
Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat testis tissue lysate with ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat testis tissue lysate
Lane 2: ab317835 IP in Rat testis tissue lysate
Lane 3: Mouse monoclonal IgG (ab18443) instead of ab317835 in rat testis tissue lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
-
![Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)](https://www.abcam.cn/ps/products/317/ab317835/Images/ab317835-7-benefitsofrecombinantantibodies.jpg)
Anti-SH3GL1/Endophilin A2 antibody [K51/1] (ab317835)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab317835 尚未被引用在任何文献中。