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重组RabMAb

重组Anti-SH2D1A/SAP抗体[EPR3168] (ab109120)

Submit a review Q&A (2)References (1)
Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
  • Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3168] to SH2D1A/SAP
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-SH2D1A/SAP抗体[EPR3168]
    参阅全部 SH2D1A/SAP 一抗

  • 描述

    兔单克隆抗体[EPR3168] to SH2D1A/SAP

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Molt-4, Jurkat, PC-12 and K562 cell lysates and mouse brain tissue lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR3168

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109120于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
说明
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

靶标

  • 功能

    Inhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.

  • 组织特异性

    Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis.

  • 疾病相关

    Defects in SH2D1A are a cause of lymphoproliferative syndrome X-linked type 1 (XLP1) [MIM:308240]; also known as X-linked lymphoproliferative disease (XLPD) or Duncan disease. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

  • 序列相似性

    Contains 1 SH2 domain.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • DSHP antibody
    • Duncan disease SH2 protein antibody
    • Duncan disease SH2-protein antibody
    • EBVS antibody
    • IMD5 antibody
    • LYP antibody
    • MTCP1 antibody
    • SAP antibody
    • SAP/SH2D1A antibody
    • SH2 domain containing 1A antibody
    • SH2 domain protein 1A antibody
    • SH2 domain-containing protein 1A antibody
    • SH21A_HUMAN antibody
    • SH2D1A antibody
    • Signaling lymphocyte activation molecule associated protein antibody
    • Signaling lymphocytic activation molecule-associated protein antibody
    • SLAM associated protein antibody
    • SLAM associated protein/SH2 domain protein 1A antibody
    • SLAM-associated protein antibody
    • T cell signal transduction molecule SAP antibody
    • T-cell signal transduction molecule SAP antibody
    • XLP antibody
    • XLPD antibody
    see all

图片

  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)

    Lane 1 : PC-12 whole cell lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)

    Lane 1 : MOLT-4 whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : K562 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/1000 dilution (unpurified)

    Lane 1 : MOLT-4 cell line lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : K562 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 14 kDa

  • Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)

文献 (1)

发表研究结果有使用 ab109120？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109120 被引用在 1 文献中.

  • Wang Y  et al. Potential pathogenic mechanism of type 1 X-linked lymphoproliferative syndrome caused by a mutation of SH2D1A gene in an infant: A case report. Medicine (Baltimore) 101:e30951 (2022). PubMed: 36254040

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Can you please provide a representative plot for the flow data for this Ab (ab109120).

Thank you.

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Answer

I now have further data to share with you in regards to the Anti-SH2D1A/SAP antibody [EPR3168] (ab109120).

I have attached the histogram produced when using the antibody to detect Molt-4 cells.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact usagain.

Read More

Question

Recently I've ordered product Anti-SH2D1A/SAP (ab109120).
Can you tell me the concentration of this antibody? I could not find it anywhere...Thank you in advance,

Read More
Answer

Thank you for your inquiry.
The concentration for ab109120, lot GR61496-3, is 0.781 mg/mL.
I hope this information helps. Please contact us with any other questions.

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