JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96029

Anti-SGSH/HSS抗体

Anti-SGSH/HSS antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal SGSH/HSS antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SGSH aa 300-500.

查看别名

HSS, SGSH, N-sulphoglucosamine sulphohydrolase, Sulfoglucosamine sulfamidase, Sulphamidase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGSH/HSS antibody (AB96029)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGSH/HSS antibody (AB96029)

Immunohistochemical analysis of formalin fixed paraffin embedded OVCAR3 xenograft, using ab96029 antibody at 1/100 dilution.

Western blot - Anti-SGSH/HSS antibody (AB96029)
  • WB

Unknown

Western blot - Anti-SGSH/HSS antibody (AB96029)

7.5% SDS Page

All lanes:

Western blot - Anti-SGSH/HSS antibody (ab96029) at 1/1000 dilution

All lanes:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 57 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human SGSH aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

P51688

style
left-column

替代产品

Primary Antibodies

AB72617

Anti-SGSH/HSS antibody

  • 0
  • 0
Primary Antibodies

AB200346

Anti-SGSH/HSS antibody [EPR17312]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

  • 4
  • 1
Primary Antibodies

AB232666

Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (AB232666)

  • 0
  • 0
Primary Antibodies

AB96030

Anti-SGSH/HSS antibody

Western blot - Anti-SGSH/HSS antibody (AB96030)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB214671

Anti-NAGLU/NAG antibody [EPR20708]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

  • 0
  • 0
Primary Antibodies

AB278043

Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker

BOND RX™ Validated|RabMAb|KO Validated|Recombinant|20ul selling size

Multiplex immunohistochemistry - Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker (AB278043)

  • 0
  • 0
Primary Antibodies

AB168341

Anti-Galactosidase alpha antibody [EP5828(2)]

RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] (AB168341)

  • 5
  • 2
Primary Antibodies

AB174844

Anti-ARSA/ASA antibody [EPR11039]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSA/ASA antibody [EPR11039] (AB174844)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The SGSH protein also known as N-sulfoglucosamine sulfohydrolase or HSS is a lysosomal enzyme with a molecular weight of approximately 56 kDa. It is primarily expressed in tissues with high turnover of glycosaminoglycans especially in the liver and brain. This enzyme localizes inside the lysosomes and plays a significant role in the degradation process of heparan sulfate. SGSH expression is important for maintaining normal cell function particularly in tissues that require constant renewal of extracellular matrix components.
Biological function summary

The enzyme acts to cleave sulfate groups from N-sulfated glucosamine residues in heparan sulfate being an important component of the degradation pathway of glycosaminoglycan. SGSH works within a lysosomal degradation complex alongside other enzymes such as iduronate-2-sulfatase and heparan-alpha-glucosaminide N-acetyltransferase each responsible for different steps in the breakdown of complex carbohydrates. Together they facilitate the complete degradation of heparan sulfate into its basic monosaccharide components.

Pathways

SGSH is integral to lysosomal degradation pathways functioning alongside proteins like alpha-L-iduronidase in the catabolic process of heparan sulfate. This breakdown pathway is essential for recycling cellular components and maintaining cellular homeostasis. The efficiencies and deficiencies in these pathways influence cellular functionality and have regulatory roles in broader metabolic circuits.

Mutations in the SGSH gene lead to Sanfilippo syndrome type IIIA also known as mucopolysaccharidosis type IIIA. This genetic disorder impairs the degradation of heparan sulfate causing an accumulation of glycosaminoglycans in cells leading to neurodegenerative conditions. In Sanfilippo syndrome type IIIA the SGSH deficiency has connections with related lysosomal storage disorders involving proteins like alpha-N-acetylglucosaminidase which further illustrate the importance of coordinated lysosomal activities.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Catalyzes a step in lysosomal heparan sulfate degradation.
See full target information SGSH
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American So 23:638-47 PubMed25592334

2015

Broad functional correction of molecular impairments by systemic delivery of scAAVrh74-hSGSH gene delivery in MPS IIIA mice.

Applications

ICC/IF

Species

Unspecified reactive species

F Jason Duncan,Bartholomew J Naughton,Kimberly Zaraspe,Darren A Murrey,Aaron S Meadows,Kelly Reed Clark,David E Newsom,Peter White,Haiyan Fu,Douglas M McCarty

Molecular pharmaceutics 11:2928-34 PubMed24949884

2014

Insulin receptor antibody-sulfamidase fusion protein penetrates the primate blood-brain barrier and reduces glycosoaminoglycans in Sanfilippo type A cells.

Applications

WB

Species

Unspecified reactive species

Ruben J Boado,Jeff Zhiqiang Lu,Eric Ka-Wai Hui,William M Pardridge
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com