Anti-SGLT2抗体[3G8] (ab58298)
Key features and details
- Mouse monoclonal [3G8] to SGLT2
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
概述
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产品名称
Anti-SGLT2抗体[3G8]
参阅全部 SGLT2 一抗 -
描述
小鼠单克隆抗体[3G8] to SGLT2 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment: GLFDKYLGAA TSLTVSEDPA VGNISSFCYR PRPDSYHLLR HPVTGDLPWP , corresponding to amino acids 228-278 of Human SGLT2
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常规说明
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
3G8 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58298于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 73 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 73 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
疾病相关
Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test. -
序列相似性
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6524 Human
- Omim: 182381 Human
- SwissProt: P31639 Human
- Unigene: 709195 Human
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别名
- Low affinity sodium glucose cotransporter antibody
- Low affinity sodium-glucose cotransporter antibody
- Na(+) glucose cotransporter 2 antibody
see all
图片
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Western blot against tagged recombinant protein immunogen using ab58298 SGLT2 antibody at 1ug/ml. Predicted band size of immunogen is 32 kDa
This image was generated using the ascites version of the product.
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Overlay histogram showing HEK293 cells stained with ab58298 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58298, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
数据表及文件
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Datasheet download
文献 (3)
ab58298 被引用在 3 文献中.
- Ngo TTT et al. Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system. Cell Prolif 55:e13190 (2022). PubMed: 35102634
- Dai C et al. Dapagliflozin does not directly affect human a or ß cells. Endocrinology N/A:N/A (2020). PubMed: 32428240
- Kuang H et al. Therapeutic Effect of Sodium Glucose Co-Transporter 2 Inhibitor Dapagliflozin on Renal Cell Carcinoma. Med Sci Monit 23:3737-3745 (2017). WB ; Human . PubMed: 28763435