Anti-SGK1 (phospho S422)抗体(ab55281)
Key features and details
- Rabbit polyclonal to SGK1 (phospho S422)
- Suitable for: WB, IHC-P, ELISA, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-SGK1 (phospho S422)抗体
参阅全部 SGK1 一抗 -
描述
兔多克隆抗体to SGK1 (phospho S422) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ELISA, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human SGK1 (phospho S422).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55281于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 49 kDa).
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 49 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
-
功能
Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1. -
组织特异性
Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
结构域
Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids. -
翻译后修饰
Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid. -
细胞定位
Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation. - Information by UniProt
-
数据库链接
- Entrez Gene: 6446 Human
- Entrez Gene: 20393 Mouse
- Entrez Gene: 29517 Rat
- Omim: 602958 Human
- SwissProt: O00141 Human
- SwissProt: Q9WVC6 Mouse
- SwissProt: Q06226 Rat
- Unigene: 510078 Human
see all -
别名
- OTTHUMP00000017247 antibody
- Serine/threonine protein kinase SGK antibody
- Serine/threonine protein kinase Sgk1 antibody
see all
图片
-
Western blot - Anti-SGK1 (phospho S422) antibody (ab55281)All lanes : Anti-SGK1 (phospho S422) antibody (ab55281) at 1/500 dilution
Lane 1 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes) plus immunizing phosphopeptide
Lane 2 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes)
Developed using the ECL technique.
Predicted band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 (phospho S422) antibody (ab55281)Immunohistochemical analysis of SGK1 (phospho S422) expression in human breast carcinoma tissue using ab55281 at a 1/50 diluton.
Left: No phosphopeptide.
Right: Sample treated with immunizing phosphopeptide.
-
Immunocytochemistry/ Immunofluorescence - Anti-SGK1 (phospho S422) antibody (ab55281)ICC/IF image of ab55281 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 4% PFA for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55281, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (26)
ab55281 被引用在 26 文献中.
- Figueroa M et al. Vascular smooth muscle cell mechanotransduction through serum and glucocorticoid inducible kinase-1 promotes interleukin-6 production and macrophage accumulation in murine hypertension. JVS Vasc Sci 4:100124 (2023). PubMed: 37920479
- Hochstetler A et al. Inhibition of serum- and glucocorticoid-induced kinase 1 ameliorates hydrocephalus in preclinical models. Fluids Barriers CNS 20:61 (2023). PubMed: 37596666
- Chen Q et al. PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2. Int J Mol Sci 23:N/A (2022). PubMed: 35955931
- Liang C et al. Homocysteine Causes Endothelial Dysfunction via Inflammatory Factor-Mediated Activation of Epithelial Sodium Channel (ENaC). Front Cell Dev Biol 9:672335 (2021). PubMed: 34222246
- Chen G et al. Monocyte Chemotactic Protein-1 Regulates Proliferation and Contractility of Human Bladder Smooth Muscle Cells Under Hydrostatic Pressure. J Interferon Cytokine Res 40:245-253 (2020). PubMed: 32091964