Anti-SGK1抗体(ab59337)
Key features and details
- Rabbit polyclonal to SGK1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-SGK1抗体
参阅全部 SGK1 一抗 -
描述
兔多克隆抗体to SGK1 -
宿主
Rabbit -
特异性
Detects endogenous levels of total SGK protein. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthesized non-phosphopeptide derived from human SGK1 around the phosphorylation site of serine 78 (P-P-SP-P-S).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| WB |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa).
|
|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa). |
|
IHC-P
1/50 - 1/100. |
靶标
-
功能
Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1. -
组织特异性
Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
结构域
Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids. -
翻译后修饰
Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid. -
细胞定位
Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation. - Information by UniProt
-
数据库链接
- Entrez Gene: 6446 Human
- Omim: 602958 Human
- SwissProt: O00141 Human
- Unigene: 510078 Human
-
别名
- OTTHUMP00000017247 antibody
- Serine/threonine protein kinase SGK antibody
- Serine/threonine protein kinase Sgk1 antibody
see all
图片
-
Western blot - Anti-SGK1 antibody (ab59337)All lanes : Anti-SGK1 antibody (ab59337) at 1/500 dilution
Lane 1 : 293 cells treated
with heat shock
Lane 2 : 293 cells treated
with heat shock with immunizing non-phosphopeptide
Predicted band size: 49 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-SGK1 antibody (ab59337)ICC/IF image of ab59337 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59337, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 antibody (ab59337)ab59337, at a 1/50-1/100 dilution, staining SGK1 in Human brain sections. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 antibody (ab59337)ab59337, at a 1/50-1/100 dilution, staining SGK1 in Human brain sections, in the presence of the immunizing peptide.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (29)
ab59337 被引用在 29 文献中.
- Tang LL et al. Aldosterone-stimulated endothelial epithelial sodium channel (EnNaC) plays a role in cold exposure-induced hypertension in rats. Front Pharmacol 13:970812 (2022). PubMed: 36278222
- Lim RG et al. Huntington disease oligodendrocyte maturation deficits revealed by single-nucleus RNAseq are rescued by thiamine-biotin supplementation. Nat Commun 13:7791 (2022). PubMed: 36543778
- Chen L et al. SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl- accumulation in respiratory epithelium. Signal Transduct Target Ther 7:255 (2022). PubMed: 35896532
- Wang Z et al. Silencing SGK1 alleviates osteoarthritis through epigenetic regulation of CREB1 and ABCA1 expression. Life Sci 268:118733 (2021). PubMed: 33171176
- Wang QS et al. NaHS or Lovastatin Attenuates Cyclosporine A-Induced Hypertension in Rats by Inhibiting Epithelial Sodium Channels. Front Pharmacol 12:665111 (2021). PubMed: 34122084