Anti-SFRS8抗体(ab72044)
Key features and details
- Rabbit polyclonal to SFRS8
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SFRS8抗体 -
描述
兔多克隆抗体to SFRS8 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human SFRS8.
Database link: Q12872 -
阳性对照
- HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72044 was affinity purified using an epitope specific to SFRS8 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72044于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 105 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 105 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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相关性
SFRS8 is a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Multiple alternatively spliced variants have been identified. Two alternatively spliced variants have been characterized to date. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 6433 Human
- Omim: 601945 Human
- SwissProt: Q12872 Human
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别名
- 1190005N23Rik antibody
- 6330437E22Rik antibody
- AI197402 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS8 antibody (ab72044)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SFRS8 with ab72044 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-SFRS8 antibody (ab72044)All lanes : Anti-SFRS8 antibody (ab72044) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 105 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation - Anti-SFRS8 antibody (ab72044)Detection of SFRS8 by Western Blot of Immunprecipitate.
ab72044 at 1µg/ml staining SFRS8 in HeLa whole cell lysate immunoprecipitated using ab72044 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab72044 尚未被引用在任何文献中。