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Anti-SFRS7/SRSF7抗体(ab72616)

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Immunocytochemistry/ Immunofluorescence - Anti-SFRS7/SRSF7 antibody (ab72616)
  • Western blot - Anti-SFRS7/SRSF7 antibody (ab72616)

Key features and details

  • Mouse polyclonal to SFRS7/SRSF7
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-SFRS7/SRSF7抗体
    参阅全部 SFRS7/SRSF7 一抗

  • 描述

    小鼠多克隆抗体to SFRS7/SRSF7

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein within Human SFRS7/SRSF7. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_001026854, 1 a.a. ~ 238 a.a)

  • 阳性对照

    • SFRS7/SRSF7 transfected lysate

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab72616于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ICC/IF
Use a concentration of 10 µg/ml.
说明
WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ICC/IF
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10.

  • 组织特异性

    Brain, liver, kidney and lung.

  • 序列相似性

    Belongs to the splicing factor SR family.
    Contains 1 CCHC-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.

  • 翻译后修饰

    Extensively phosphorylated on serine residues in the RS domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 9G8 antibody
      • AAG3 antibody
      • arginine/serine-rich 7 antibody
      • HSSG1 antibody
      • RBM37 antibody
      • Serine/arginine-rich splicing factor 7 antibody
      • Splicing factor 9G8 antibody
      • Splicing factor antibody
      • Splicing factor, arginine/serine rich 7 antibody
      • SRSF7 antibody
      • SRSF7_HUMAN antibody
      • ZCCHC20 antibody
      • ZCRB2 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-SFRS7/SRSF7 antibody (ab72616)
      Immunocytochemistry/ Immunofluorescence - Anti-SFRS7/SRSF7 antibody (ab72616)

      Immunocytochemistry of HeLa cells staining SFRS7/SRSF7 using ab72616 at 10 μg/ml.

    • Western blot - Anti-SFRS7/SRSF7 antibody (ab72616)
      Western blot - Anti-SFRS7/SRSF7 antibody (ab72616)
      All lanes : Anti-SFRS7/SRSF7 antibody (ab72616) at 1/500 dilution

      Lane 1 : SFRS7/SRSF7 transfected 293T cell line
      Lane 2 : Non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 27 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?

    文献 (0)

    发表研究结果有使用 ab72616？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab72616 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    I purchased this antibody (ab72616) from you in November 2011 but I have not been able to get any signal using it for western blotting at the dilutions stated (1/500 or 1/1000) on human cell line lysates. Please can you tell me if there any specific techniques I can try to get a signal, or failing that how I can get a refund through the Abpromise scheme advertised on your website?

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    Answer

    Thank you for bringing this to our attention. I am sorry to read that you are having difficulty obtaining a signal in your blots.

    If you do not already check for effective transfer, I recommend doing so with a Ponceau Red stain on a new blot. If you are confident that transfer was good, then I recommend stripping an existing blot, re-blocking with 1% BSA, and diluting the antibody in the same blocking solution, with no more than 1% BSA, using a diution of 1/100. The 1/500 that the datasheet recommends was based on the validation with a transfected cell lysate. As you can see on the datasheet, the antibody was incapable of detecting endogenous SFRS7 in a lysate of 293T cells at 1/500. That may be the case for your cells too.

    Are you confident that your samples contain SFRS7? If not, I can recommend a positive control

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