重组Anti-SFRP2抗体[EPR26088-157] (ab318185)
![Western blot - Anti-SFRP2 antibody [EPR26088-157] (ab318185)](https://www.abcam.cn/ps/products/318/ab318185/Images/ab318185-542846-anti-sfrp2-antibody-epr26088-157-western-blot-human-eyeball-tissue-lysate30-ug.jpg "Western blot - Anti-SFRP2 antibody [EPR26088-157] (ab318185)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26088-157] to SFRP2
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SFRP2抗体[EPR26088-157]
参阅全部 SFRP2 一抗 -
描述
兔单克隆抗体[EPR26088-157] to SFRP2 -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human eyeball tissue, Human breast tissue, Untreated Y79 whole cell and Y79 treated with 300ng/ml Brefeldin A for 24 hours whole cell lysates. Dot: His-tagged human SFRP2 recombinant protein.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR26088-157 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318185于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB |
1/1000. Predicted molecular weight: 33 kDa.
|
| 说明 |
|---|
|
Dot blot
1/1000. |
|
WB
1/1000. Predicted molecular weight: 33 kDa. |
靶标
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功能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. -
组织特异性
Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. -
序列相似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
结构域
The FZ domain is involved in binding with Wnt ligands. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6423 Human
- Omim: 604157 Human
- SwissProt: Q96HF1 Human
- Unigene: 481022 Human
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别名
- AI851596 antibody
- Frizzled related protein 2 antibody
- FRP 2 antibody
see all
图片
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Western blot - Anti-SFRP2 antibody [EPR26088-157] (ab318185)All lanes : Anti-SFRP2 antibody [EPR26088-157] (ab318185) at 1/1000 dilution
Lane 1 : Human eyeball tissue lysate
Lane 2 : Human breast tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Untreated Y79 (human retinoblastoma cell) whole cell lysate
Lane 5 : Y79 treated with 300ng/ml Brefeldin A for 24 hours whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: spleen.
Lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Dot Blot - Anti-SFRP2 antibody [EPR26088-157] (ab318185)](https://www.abcam.cn/ps/products/318/ab318185/Images/ab318185-2-anti-sfrp2-antibody-epr26088-157-dot-blot-his-tagged-human-sfrp2-recombinant-protein.jpg)
Dot Blot - Anti-SFRP2 antibody [EPR26088-157] (ab318185)Dot blot analysis of SFRP2 using ab318185 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Lane 1: His-tagged human SFRP2 recombinant protein
Lane 2: His-tagged human SFRP1 recombinant proteinExposure time: 180 seconds..
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human SFRP1.
Anti-6X His tag ® antibody [EPR20547] - ChIP Grade (ab213204) (1:1000) as total protein control. -
![Anti-SFRP2 antibody [EPR26088-157] (ab318185)](https://www.abcam.cn/ps/products/318/ab318185/Images/ab318185-3-benefitsofrecombinantantibodies.jpg)
Anti-SFRP2 antibody [EPR26088-157] (ab318185)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab318185 尚未被引用在任何文献中。