Anti-SF3B2抗体[5D2] (ab56800)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (ab56800)](https://www.abcam.cn/ps/products/56/ab56800/Images/ab56800-70277-anti-sf3b2-antibody-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (ab56800)")
Key features and details
- Mouse monoclonal [5D2] to SF3B2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-SF3B2抗体[5D2] -
描述
小鼠单克隆抗体[5D2] to SF3B2 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: ISLGMPVGPN AHKVPPPWLI AMQRYGPPPS YPNLKIPGLN SPIPESCSFG YHAGG, corresponding to amino acids 592-646 of Human SF3B2
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常规说明
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4 -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
5D2 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab56800于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
SF3B2 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B2 may also be involved in the assembly of the 'E' complex. It also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 10992 Human
- Omim: 605591 Human
- SwissProt: Q13435 Human
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别名
- Pre mRNA splicing factor SF3b 145 kDa subunit antibody
- SAP 145 antibody
- SAP145 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (ab56800)
SF3B2 antibody (ab56800) used in immunohistochemistry at 6ug/ml on formalin fixed and paraffin embedded human kidney.
This image was generated using the ascites version of the product.
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![Western blot - Anti-SF3B2 antibody [5D2] (ab56800)](https://www.abcam.cn/ps/products/56/ab56800/Images/ab56800-70276-anti-sf3b2-antibody-western-blot.jpg)
Western blot - Anti-SF3B2 antibody [5D2] (ab56800)SF3B2 antibody (ab56800) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-SF3B2 antibody [5D2] (ab56800)](https://www.abcam.cn/ps/products/56/ab56800/Images/ab56800-70275-anti-sf3b2-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SF3B2 antibody [5D2] (ab56800)ab56800, at 60 µg/ml staining SF3B2 in HeLa Cells by immunocytochemistry. Immunofluorescence of ab56800 to SF3B2 on HeLa cell, performed with antibody concentration at 60 ug/ml.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab56800 被引用在 3 文献中.
- Simmler P et al. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer. Cell Rep 40:111266 (2022). PubMed: 36001976
- Murthy T et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
- Lin-Moshier Y et al. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem 288:355-67 (2013). WB . PubMed: 23148228
