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重组RabMAb

重组Anti-SF3B1抗体[EPR11986] (ab172634)

Reviews (1) Submit a question References (14)
Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Anti-SF3B1 antibody [EPR11986] (ab172634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11986] to SF3B1
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free HRP PE

概述

  • 产品名称

    Anti-SF3B1抗体[EPR11986]
    参阅全部 SF3B1 一抗

  • 描述

    兔单克隆抗体[EPR11986] to SF3B1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human SF3B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75533

  • 阳性对照

    • WB: HeLa, Jurkat, K562 and Ramos cell lysates. IHC-P: Human colon and glioma tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR11986

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab172634于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/2000.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
ICC/IF
1/200 - 1/500.
说明
Flow Cyt (Intra)
1/2000.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
ICC/IF
1/200 - 1/500.
应用说明
Is unsuitable for IP.

靶标

  • 相关性

    SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.

  • 细胞定位

    Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

  • 数据库链接

    • 别名

      • Hsh 155 antibody
      • MDS antibody
      • Pre mRNA processing 10 antibody
      • Pre mRNA splicing factor SF3b 155 kDa subunit antibody
      • PRP 10 antibody
      • PRP10 antibody
      • PRPF 10 antibody
      • PRPF10 antibody
      • SAP 155 antibody
      • SAP155 antibody
      • SF3B 1 antibody
      • SF3b155 antibody
      • Spliceosome associated protein 155 antibody
      • Splicing factor 3b subunit 1 155kDa antibody
      • Splicing factor 3B subunit 1 antibody
      see all

    图片

    • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution

      Lane 1 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 146 kDa
      Observed band size: 146 kDa



      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      Exposure time:
      Lane 1-3: 3.25 seconds
      Lane 4: 15 seconds

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) labeling SF3B1 with ab172634 at 1/200 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain antibody.

      Confocal image showing nuclear staining in NIH/3T3 cell line.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervical adenocarcinoma epithelial cell) labeling SF3B1 with ab172634 at 1/200 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain antibody.

      Confocal image showing nuclear staining in Hela cell line.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 

    • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution

      Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) nuclear fraction
      Lane 2 : HeLa (human cervical adenocarcinoma epithelial cell) cytoplasmic fraction
      Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell) nuclear fraction
      Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) cytoplasmic fraction
      Lane 5 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) nuclear fraction
      Lane 6 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cytoplasmic fraction

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 146 kDa
      Observed band size: 145 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      Exposure time:
       Lane 1-4: 1 second
      Lane 5-6: 10 seconds

      In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution and anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

    • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Jurkat lysate
      Lane 3 : K562 lysate
      Lane 4 : Ramos lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 146 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-SF3B1 antibody [EPR11986] (ab172634)
      Anti-SF3B1 antibody [EPR11986] (ab172634)

    文献 (14)

    发表研究结果有使用 ab172634？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab172634 被引用在 14 文献中.

    • Fuentes-Fayos AC  et al. SF3B1 inhibition disrupts malignancy and prolongs survival in glioblastoma patients through BCL2L1 splicing and mTOR/ß-catenin pathways imbalances. J Exp Clin Cancer Res 41:39 (2022). PubMed: 35086552
    • Simmler P  et al. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer. Cell Rep 40:111266 (2022). PubMed: 36001976
    • Zhang M  et al. Neural stemness unifies cell tumorigenicity and pluripotent differentiation potential. J Biol Chem 298:102106 (2022). PubMed: 35671824
    • Rouf Banday A  et al. Targeting natural splicing plasticity of APOBEC3B restricts its expression and mutagenic activity. Commun Biol 4:386 (2021). PubMed: 33753867
    • Yang JY  et al. SF3B1 mutation in pancreatic cancer contributes to aerobic glycolysis and tumor growth through a PP2A-c-Myc axis. Mol Oncol 15:3076-3090 (2021). PubMed: 33932092
    View all Publications for this product

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    ChIP abreview for Anti-SF3B1 antibody [EPR11986]

     Poor
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Negative control
    Epsilon globin gene
    Specification
    Jurkat cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 40 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 30min 1.5mM EGS 10 min 1% HCHO
    Positive control
    PCNA gene
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 Sep 21 2015

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