AB156873

重组Anti-SF3A3抗体[EPR10020]

Anti-SF3A3 antibody [EPR10020]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal SF3A3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

查看别名

SAP61, SF3A3, Splicing factor 3A subunit 3, SF3a60, Spliceosome-associated protein 61, SAP 61

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] (AB156873)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] (AB156873)

Immunofluorescent analysis of Hela cells labeling SF3A3 with ab156873 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

Immunohistochemical analysis of formalin-fixed paraffin-embedded lung carcinoma tissue labelling SF3A3 with ab156873 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

ab156873 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

ab156873 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

ab156873 showing +ve staining in Human cervical carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

ab156873 showing +ve staining in Human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (AB156873)

ab156873 showing +ve staining in Human breast carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SF3A3 antibody [EPR10020] (AB156873)

WB

Unknown

Western blot - Anti-SF3A3 antibody [EPR10020] (AB156873)

All lanes:

Western blot - Anti-SF3A3 antibody [EPR10020] (ab156873) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 59 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-SF3A3 antibody [EPR10020] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10020

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, WB, IHC-P

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SF3A3 also known as Splicing Factor 3A Subunit 3 plays an important role in pre-mRNA splicing. It has a mass of approximately 60 kDa. SF3A3 is a component of the splicing machinery and localizes predominantly to the nucleus where it is involved in the assembly of the spliceosome. The gene encoding SF3A3 is expressed in a wide range of tissues indicating its fundamental role in the splicing process across different cell types.

Biological function summary

SF3A3 is part of the U2 small nuclear ribonucleoprotein (snRNP) complex which is important for the second step of the splicing process. This complex recognizes the pre-mRNA branch site and participates in the assembly of the spliceosome a complex that excises introns and joins exons to produce mature mRNA. The activity of SF3A3 is essential for the accurate splicing of precursor mRNA transcripts impacting gene expression regulation.

Pathways

SF3A3 plays a role in the mRNA processing pathway and the spliceosome pathway. These pathways are vital for gene expression regulation and the generation of functional RNA products. Within the spliceosome pathway SF3A3 is associated with other splicing factors such as SF3B1 which also plays a role in splicing regulation and influences the assembly and function of the spliceosome complex.

SF3A3 has been linked to cancer and myelodysplastic syndromes. Mutations or dysregulation of SF3A3 and its associated splicing factors like SF3B1 can lead to aberrant splicing which may contribute to oncogenesis and disease progression. Understanding SF3A3's role in these disorders could provide insights into therapeutic targets for treatment strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006, PubMed : 34822310, PubMed : 8022796). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex (PubMed : 10882114, PubMed : 11533230). Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes (PubMed : 29360106, PubMed : 30315277).

See full target information SF3A3

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 34:119-135.e10 PubMed29937354

2018

Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Marat S Pavlyukov,Hai Yu,Soniya Bastola,Mutsuko Minata,Victoria O Shender,Yeri Lee,Suojun Zhang,Jia Wang,Svetlana Komarova,Jun Wang,Shinobu Yamaguchi,Heba Allah Alsheikh,Junfeng Shi,Dongquan Chen,Ahmed Mohyeldin,Sung-Hak Kim,Yong Jae Shin,Ksenia Anufrieva,Evgeniy G Evtushenko,Nadezhda V Antipova,Georgij P Arapidi,Vadim Govorun,Nikolay B Pestov,Mikhail I Shakhparonov,L James Lee,Do-Hyun Nam,Ichiro Nakano

Cancer research 78:3190-3206 PubMed29700004

2018

PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Zi Zheng,Meng-Zhu Xue,Hong-Jie Shen,Xiao-Guang Li,Ding Ma,Yue Gong,Yi-Rong Liu,Feng Qiao,Hong-Yan Xie,Bi Lian,Wei-Li Sun,Hai-Yun Zhao,Ling Yao,Wen-Jia Zuo,Da-Qiang Li,Peng Wang,Xin Hu,Zhi-Ming Shao

The Journal of cell biology 209:235-46 PubMed25918225

2015

Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing.

Applications

IP, WB

Species

Unspecified reactive species, Unspecified reactive species

Yihan Wan,Xiaobin Zheng,Haiyang Chen,Yuxuan Guo,Hao Jiang,Xiaonan He,Xueliang Zhu,Yixian Zheng

Molecular & cellular proteomics : MCP 13:3585-601 PubMed25277244

2014

The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets.

Applications

WB

Species

Unspecified reactive species

Maria Katsogiannou,Claudia Andrieu,Virginie Baylot,Anaïs Baudot,Nelson J Dusetti,Odile Gayet,Pascal Finetti,Carmen Garrido,Daniel Birnbaum,François Bertucci,Christine Brun,Palma Rocchi

View all publications

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