JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB129108

Anti-SF2 抗体 [EPR8239]

Anti-SF2 antibody [EPR8239]

2

(2 Reviews)

|

(18 Publications)

Rabbit Recombinant Monoclonal SF2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 18 publications.

查看别名

ASF, SF2, SF2P33, SFRS1, OK/SW-cl.3, SRSF1, Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SF2 with unpurified ab129108 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8239] (AB129108)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8239] (AB129108)

Intracellular Flow Cytometry analysis of HeLa cells labelling SF2 with purified ab129108 at a dilution of 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8239] (AB129108)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8239] (AB129108)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SF2 with purified ab129108 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Counterstained with DAPI.

Control - primary antibody (1/150) secondary antibody ab150120 an Alexa Fluor® 594-conjugate goat anti-mouse IgG (1/500).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Western blot - Anti-SF2 antibody [EPR8239] (AB129108)
  • WB

Lab

Western blot - Anti-SF2 antibody [EPR8239] (AB129108)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SF2 antibody [EPR8239] (ab129108) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 28 kDa

false

Western blot - Anti-SF2 antibody [EPR8239] (AB129108)
  • WB

Lab

Western blot - Anti-SF2 antibody [EPR8239] (AB129108)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SF2 antibody [EPR8239] (ab129108) at 1/10000 dilution

All lanes:

Mouse spleen tissue at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 28 kDa

false

OI-RD Scanning - Anti-SF2 antibody [EPR8239] (AB129108)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SF2 antibody [EPR8239] (AB129108)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (10)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8239

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800 - 1/1500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800 - 1/1500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800 - 1/1500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Stable for 12 months at -20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SF2 also known as Splicing Factor 2 or Alternative Splicing Factor 1 (ASF1) is a prototypical member of the SR protein family involved in mRNA splicing. It is approximately 33 kDa in mass. SF2 is expressed ubiquitously in the nucleus of eukaryotic cells where it plays a central role in the regulation of constitutive and alternative splicing processes.
Biological function summary

SF2 acts as an important regulator of gene expression by facilitating the splicing of pre-mRNA participating in spliceosome assembly. It interacts with other splicing factors and RNA to form spliceosomal complexes essential for splicing fidelity and efficiency. The precise modulation of splicing by SF2 allows for the generation of diverse protein isoforms from a single gene contributing to protein diversity within the cell.

Pathways

SF2 integrates into the RNA splicing pathway and is critical for splice site selection. It interacts with proteins such as U1 snRNP and U2AF65 influencing their binding to pre-mRNA. SF2 also plays roles in other key pathways like mRNA transport and translation thereby linking the splicing machinery with nuclear export and protein synthesis processes.

Abnormalities in SF2 levels or functions have been linked to various cancers and spinal muscular atrophy (SMA). Cancer cells often show altered SF2 expression leading to splicing anomalies that promote tumorigenesis. Additionally SF2's interaction with proteins like hnRNP A1 influences disease mechanisms as splicing factor dysregulation can disrupt normal cellular processes and facilitate the progression of disorders such as SMA.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
See full target information SRSF1
style
left-column

文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:25 PubMed39837814

2025

Targeting SRSF1 improves cancer immunotherapy by dually acting on CD8T and tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Qi Zhu,Zheng Tang,Tian-Hao Chu,Biao Wang,Shi-Ping Chen,Chen-Yang Tao,Jia-Liang Cai,Rui Yang,Wei-Feng Qu,Yi Wang,Qian-Fu Zhao,Run Huang,Meng-Xin Tian,Yuan Fang,Jun Gao,Xiao-Ling Wu,Jian Zhou,Wei-Ren Liu,Zhi Dai,Ying-Hong Shi,Jia Fan

Cytotechnology 76:313-327 PubMed38736729

2024

LncRNA MIR181A2HG negatively regulates human keratinocytes proliferation by binding SRSF1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomei Fan,Mingzhao Li,Mutian Niu,Fangru Chen,Zhijing Mo,Pengpeng Yue,Mengjiao Wang,Qingbo Liu,Bin Liang,Shaoqin Gan,Chengke Weng,Jintao Gao

Nature structural & molecular biology 31:1341-1354 PubMed38658621

2024

Alternative splicing of CARM1 regulated by LincGET-guided paraspeckles biases the first cell fate in mammalian early embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqiang Wang,Yiwei Zhang,Jiaze Gao,Guihai Feng,Chao Liu,Xueke Li,Pengcheng Li,Zhonghua Liu,Falong Lu,Leyun Wang,Wei Li,Qi Zhou,Yusheng Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2301107 PubMed37097746

2023

Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Sun,Panpan Ji,Tian Zhou,Zhelong Li,Changyang Xing,Liang Zhang,Mengying Wei,Guodong Yang,Lijun Yuan

Aging 15:1025-1038 PubMed36800233

2023

Circ_0001052 promotes cardiac hypertrophy via elevating Hipk3.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyue Yang,Weichen Wang,Longlong Wang,Yuze Li

American journal of physiology. Heart and circulatory physiology 322:H1014-H1027 PubMed35302878

2022

Serine-arginine-rich protein kinase-1 inhibition for the treatment of diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Naseeb K Malhi,Claire L Allen,Elizabeth Stewart,Katherine L Horton,Federica Riu,Jennifer Batson,Winfried Amoaku,Jonathan C Morris,Kenton P Arkill,David O Bates

Nature communications 12:4980 PubMed34404792

2021

Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Samuel A Myers,Dominique K Carey,Steven A Carr,Alice Y Ting

Neuron 109:1675-1691.e9 PubMed33848474

2021

Tau aggregates are RNA-protein assemblies that mislocalize multiple nuclear speckle components.

Applications

Unspecified application

Species

Unspecified reactive species

Evan Lester,Felicia K Ooi,Nadine Bakkar,Jacob Ayers,Amanda L Woerman,Joshua Wheeler,Robert Bowser,George A Carlson,Stanley B Prusiner,Roy Parker

American journal of physiology. Lung cellular and molecular physiology 320:L726-L738 PubMed33565360

2021

Matrix stiffening induces a pathogenic QKI-miR-7-SRSF1 signaling axis in pulmonary arterial endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Shan Chen Woodcock,Neha Hafeez,Adam Handen,Ying Tang,Lloyd D Harvey,Leonard E Estephan,Gil Speyer,Seungchan Kim,Thomas Bertero,Stephen Y Chan

Molecular cancer 17:157 PubMed30419914

2018

Sunitinib-suppressed miR-452-5p facilitates renal cancer cell invasion and metastasis through modulating SMAD4/SMAD7 signals.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhai,Saiyang Li,Jin Zhang,Yonghui Chen,Junjie Ma,Wen Kong,Dongkui Gong,Junhua Zheng,Wei Xue,Yunfei Xu
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB238523

Anti-SF2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody (AB238523)

  • 0
  • 0
Primary Antibodies

AB133689

Anti-SF2 antibody [EPR8240]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8240] (AB133689)

  • 5
  • 1
Primary Antibodies

AB207540

Alexa Fluor® 647 Anti-SF2 antibody [EPR8240]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SF2 antibody [EPR8240] (AB207540)

  • 0
  • 0
Primary Antibodies

AB38017

Anti-SF2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody (AB38017)

  • 4
  • 6

搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB133607

Anti-hnRNP C1/C2 antibody [EPNCIR152]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)

  • 5
  • 2
Primary Antibodies

AB198291

Anti-SRSF3 antibody [EPR16976]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SRSF3 antibody [EPR16976] (AB198291)

  • 5
  • 6

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com