AB236027

重组Anti-SESN1抗体[EPR1930(2)] - BSA and Azide free

Anti-SESN1 antibody [EPR1930(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SESN1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

查看别名

PA26, SEST1, SESN1, Sestrin-1, p53-regulated protein PA26

2 Images

Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] - BSA and Azide free (AB236027)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SESN1 antibody [EPR1930(2)] - BSA and Azide free (AB236027)

Intracellular Flow Cytometry analysis of permeabilized K562 cells labeling SESN1 with ab134091 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134091).

OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] - BSA and Azide free (AB236027)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SESN1 antibody [EPR1930(2)] - BSA and Azide free (AB236027)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Unconjugated

Anti-SESN1 antibody [EPR1930(2)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1930(2)

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab236027 is the carrier-free version of ab134091.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SESN1 also known as Sestrin-1 is a protein that has significant significance in cellular stress responses. It has a molecular weight of approximately 57 kDa. This protein mainly expresses in tissues such as liver skin and heart. Sestrin-1 acts as a regulator of cellular homeostasis modulating factors tied to oxidative stress correction. The protein carries antioxidant properties mediating the regeneration of peroxiredoxins which are important for detoxifying peroxides within cells.

Biological function summary

The Sestrin-1 protein plays a role in the cellular defense against oxidative damage. It incorporates into complexes that participate in inhibiting the activity of the mechanistic target of rapamycin complex 1 (mTORC1) a critical regulator of cell growth and metabolism. Sestrin-1 achieves this by activating AMP-activated protein kinase (AMPK) which serves as an energy sensor in the cell. Additionally it can suppress free radical accumulation during periods of cellular stress helping to prevent damage to DNA and proteins.

Pathways

Sestrin-1 is a vital component of the signaling pathways that involve the AMPK and mTOR pathways. It interacts actively with proteins such as TSC2 in mTOR signaling and LKB1 in the AMPK pathway. Through these pathways Sestrin-1 influences the regulation of protein synthesis and autophagy. It acts as a link between energy status and cellular growth control underpinning its involvement in adaptive cellular responses to environmental stresses.

Sestrin-1 involvement connects to conditions like cancer and neurodegenerative diseases. In cancer its role in inhibiting the mTORC1 pathway gives it potential anti-tumorigenic properties as overactive mTOR signaling can lead to uncontrolled cell proliferation. Additionally in neurodegenerative disorders Sestrin-1 reduces stress-induced neuronal damage through its antioxidant functions. Sestrin-1 interacts with proteins like p53 a well-known tumor suppressor to aid in cellular protective mechanisms providing a link between its protective role and disease suppression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed : 25263562, PubMed : 26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed : 23274085). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed : 15105503). However, this could not be confirmed (By similarity).

See full target information SESN1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiovascular pharmacology 80:609-615 PubMed35881902

2022

Sestrin2 Is Increased in Calcific Aortic Disease and Inhibits Osteoblastic Differentiation in Valvular Interstitial Cells via the Nuclear Factor E2-related Factor 2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haixiong Wang,Jicheng Xi,Zhibiao Zhang,Jun Li,Liping Guo,Na Li,Yuehui Sun,Xiaofang Li,Xuebin Han

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-SESN1抗体[EPR1930(2)] - BSA and Azide free