重组Anti-SESN1抗体[EPR1930(2)] (ab134091)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1930(2)] to SESN1
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SESN1抗体[EPR1930(2)]
参阅全部 SESN1 一抗 -
描述
兔单克隆抗体[EPR1930(2)] to SESN1 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SESN1 aa 100-200 (internal sequence). The exact sequence is proprietary.
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阳性对照
- WB: Human heart, Ramos, K-562, JAR, HeLa, F9, C2C12, NIH/3T3, and Rat heart lysates. Flow Cyt (intra): Daudi and permeabilized K562 cells,
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.08 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1930(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134091于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the sestrin family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 27244 Human
- Entrez Gene: 140742 Mouse
- Entrez Gene: 294518 Rat
- Omim: 606103 Human
- SwissProt: Q9Y6P5 Human
- SwissProt: P58006 Mouse
- Unigene: 591336 Human
- Unigene: 139418 Mouse
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别名
- 1110002G11Rik antibody
- AU044290 antibody
- MGC118148 antibody
see all
图片
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All lanes : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution (Purified)
Lane 1 : Human heart lysates
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 3 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?The bands observed are consistent with what has been described in PMID: 29751091 and PMID: 29263116
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Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells treated with 20 U/ml IFN alpha 1 for 24 hours (Red) / Untreated control (Green) labelling Anti-SESN1 with unpurified ab207414 at 1/600 dilution.Cells were fixed with 2% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Black - isotype control, Rabbit monoclonal IgG (ab172730). Blue -unlabelled control,cells without incubation with primary and secondary antibodies.
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Lanes 1 & 3-7 : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution ((unpurified))
Lane 2 : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : NIH3T3 cell lysate
Lane 6 : F9 cell lysate
Lane 7 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
Intracellular Flow Cytometry analysis of permeabilized K562 cells labeling SESN1 with unpurified ab134091 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab134091 被引用在 11 文献中.
- Yang F et al. Effects of ferroptosis in myocardial ischemia/reperfusion model of rat and its association with Sestrin 1. Adv Clin Exp Med 32:219-231 (2023). PubMed: 36413176
- Lanna A et al. An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory. Nat Cell Biol 24:1461-1474 (2022). PubMed: 36109671
- Hayasaka T et al. Sarcopenia-derived exosomal micro-RNA 16-5p disturbs cardio-repair via a pro-apoptotic mechanism in myocardial infarction in mice. Sci Rep 11:19163 (2021). PubMed: 34580402
- Sun Y et al. Aerobic exercise inhibits inflammatory response in atherosclerosis via Sestrin1 protein. Exp Gerontol 155:111581 (2021). PubMed: 34634412
- Ge L et al. Sestrin3 enhances macrophage-mediated generation of T helper 1 and T helper 17 cells in a mouse colitis model. Int Immunol 32:421-432 (2020). PubMed: 32154559