Anti-Serum Response Factor SRF抗体(ab53147)
Key features and details
- Rabbit polyclonal to Serum Response Factor SRF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Serum Response Factor SRF抗体
参阅全部 Serum Response Factor SRF 一抗 -
描述
兔多克隆抗体to Serum Response Factor SRF -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic non phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 99 (S-L-S-E-M).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53147 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53147于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/300 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/300 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation. -
序列相似性
Contains 1 MADS-box domain. -
翻译后修饰
Phosphorylated by PRKDC. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6722 Human
- Entrez Gene: 20807 Mouse
- Omim: 600589 Human
- SwissProt: P11831 Human
- SwissProt: Q9JM73 Mouse
- Unigene: 520140 Human
- Unigene: 45044 Mouse
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别名
- c fos serum response element binding factor antibody
- c fos serum response element binding transcription factor antibody
- ELK3 antibody
see all
图片
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Western blot - Anti-Serum Response Factor SRF antibody (ab53147)All lanes : Anti-Serum Response Factor SRF antibody (ab53147) at 1/300 dilution
Lane 1 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with no peptide
Lane 2 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with peptide
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody (ab53147)Immunohistochemical analysis of Serum Response Factor SRF in paraffin embedded human breast carcinoma tissue using ab53147 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab53147 被引用在 15 文献中.
- Bartosova L et al. Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats. Front Endocrinol (Lausanne) 13:1029750 (2022). PubMed: 36568083
- Kanemaru H et al. A mechanism of cooling hot tumors: Lactate attenuates inflammation in dendritic cells. iScience 24:103067 (2021). PubMed: 34541473
- Li B et al. Long non-coding RNA H19 contributes to wound healing of diabetic foot ulcer. J Mol Endocrinol N/A:N/A (2020). PubMed: 32698141
- Zhang Y et al. LncRNA XIST modulates 5-hydroxytrytophan-induced visceral hypersensitivity by epigenetic silencing of the SERT gene in mice with diarrhea-predominant IBS. Cell Signal N/A:109674 (2020). PubMed: 32446903
- Liu Y et al. lncRNA CYTOR promotes tamoxifen resistance in breast cancer cells via sponging miR-125a-5p. Int J Mol Med 45:497-509 (2020). PubMed: 31894257