重组Anti-Serum Amyloid P/SAP抗体[EP1018Y] (ab45151)
![Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.cn/ps/products/45/ab45151/Images/ab45151-266211-anti-serum-amyloid-p-sap-antibody-ep1018y-western-blot.jpg "Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Serum Amyloid P/SAP抗体[EP1018Y]
参阅全部 Serum Amyloid P/SAP 一抗 -
描述
兔单克隆抗体[EP1018Y] to Serum Amyloid P/SAP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Serum Amyloid P/SAP aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: Human serum; Human liver lysate. IHC-P: Human endometrial carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1018Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45151于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
|
| IHC-P |
1/50 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results |
| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
|
IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results |
靶标
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功能
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. -
组织特异性
Found in serum and urine. -
疾病相关
Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. -
序列相似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
翻译后修饰
N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 325 Human
- Omim: 104770 Human
- SwissProt: P02743 Human
- Unigene: 507080 Human
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别名
- 9.5S alpha 1 glycoprotein antibody
- 9.5S alpha-1-glycoprotein antibody
- Amyloid P component serum antibody
see all
图片
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Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/10000 dilution + Human serum at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDaDiluting and blocking buffer: 5% NFDM /TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.cn/ps/products/45/ab45151/Images/ab45151-266212-anti-serum-amyloid-p-sap-antibody-ep1018y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)ab45151 staining Serum Amyloid P/SAP in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
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![Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.cn/ps/products/45/ab45151/Images/ab45151-266210-anti-serum-amyloid-p-sap-antibody-ep1018y-western-blot.jpg)
Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/5000 dilution + Human liver at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 25 kDaBlocking and diluting buffer: 5% NFDM /TBST
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![Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.cn/ps/products/45/ab45151/Images/ab45151-6-benefits-of-recombinant-antibodies.png)
Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab45151 被引用在 11 文献中.
- Lee JH et al. Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis. Clin Proteomics 17:20 (2020). PubMed: 32518534
- Bergkvist L et al. Serum amyloid P component promotes formation of distinct aggregated lysozyme morphologies and reduces toxicity in Drosophila flies expressing F57I lysozyme. PLoS One 15:e0227227 (2020). PubMed: 31978114
- Bai B et al. Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease Progression. Neuron 105:975-991.e7 (2020). PubMed: 31926610
- Girton AW et al. Serum Amyloid P and IgG Exhibit Differential Capabilities in the Activation of the Innate Immune System in Response to Bacillus anthracis Peptidoglycan. Infect Immun 86:N/A (2018). PubMed: 29531132
- Helmfors L et al. Serum Amyloid P Component Ameliorates Neurological Damage Caused by Expressing a Lysozyme Variant in the Central Nervous System of Drosophila melanogaster. PLoS One 11:e0159294 (2016). IHC-P . PubMed: 27428539