Anti-Serum Amyloid P/SAP抗体[14B4] (ab27313)
![Western blot - Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313)](https://www.abcam.cn/ps/products/27/ab27313/Images/ab27313-485043-ab27313-AntiSerum-Amyloid-PSAP-antibody-14B4-western-blot-SAP-unreduced-and-reduced.jpg "Western blot - Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313)")
Key features and details
- Mouse monoclonal [14B4] to Serum Amyloid P/SAP
- Suitable for: Sandwich ELISA, WB, ELISA
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Serum Amyloid P/SAP抗体[14B4]
参阅全部 Serum Amyloid P/SAP 一抗 -
描述
小鼠单克隆抗体[14B4] to Serum Amyloid P/SAP -
宿主
Mouse -
特异性
None detected so far. -
经测试应用
适用于: Sandwich ELISA, WB, ELISAmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Serum Amyloid P/SAP.
Database link: P02743 -
常规说明
This antibody reacts with Serum Amyloid P / SAP coated on microtitre plates.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
纯度
Protein A purified -
Primary antibody说明
This antibody reacts with serum Amyloid P coated on microtitre plates. In western blots of SDS-PAGE separated serum proteins, the antibody reacts with a high molecular weight aggregate of serum Amyloid P under non reducing conditions, and reacts strongly with a 25 kDa band of the single subunits under reducing SDS-PAGE conditions. -
克隆
单克隆 -
克隆编号
14B4 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27313于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
Use as Capture antibody with recommended pair. See Abreview submitted by Jennifer Webster for details. |
|
| WB | (1) |
Use at an assay dependent concentration.
|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Use as Capture antibody with recommended pair. See Abreview submitted by Jennifer Webster for details. |
|
WB
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
靶标
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功能
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. -
组织特异性
Found in serum and urine. -
疾病相关
Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. -
序列相似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
翻译后修饰
N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 325 Human
- Omim: 104770 Human
- SwissProt: P02743 Human
- Unigene: 507080 Human
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别名
- 9.5S alpha 1 glycoprotein antibody
- 9.5S alpha-1-glycoprotein antibody
- Amyloid P component serum antibody
see all
图片
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Western blot - Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313)Lanes 2 & 4 : Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313) at 2 µg/ml
Lanes 1 & 3 : molecular weight market
Lane 2 : human serum amyloid P component, unreduced at 0.2 µg
Lane 4 : human serum amyloid P component, reduced at 0.2 µg
Exposure time: 60 secondsIn each well of a Novex Wedge well 4-20% Tris-Glycine gel, 0.2 µg of human serum amyloid P component was loaded in a volume of 10 µL of either non-reducing or reducing loading buffer.
A nitrocellulose membrane was used for blotting. The membrane was blocked overnight in 50 mL of PBS supplemented with 0.1% BSA and 0.1% Tween 20.
The nitrocellulose membrane was incubated for 1 h with 20 mL of 2 µg/mL ab27313 Anti Serum Amyloid P component (SAP), human diluted in PBS supplemented with 0.1% BSA and 0.1% Tween 20.
After washing for 3 ×5 min in 50 mL PBS supplemented with 0.1% BSA and 0.1% Tween 20, the membrane was incubated for 1 h with rabbit anti mouse IgG diluted 1: 2000 and Streptactin-HRP diluted 1:2000 in 10 ml of PBS supplemented with 0.1% BSA and 0.1% Tween 20.
After another washing for 3 ×5 min in 50 mL PBS supplemented with 0.1% BSA and 0.1% Tween 20, the membrane was incubated for 5 min in the dark with Clarity Western ECL.
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![ELISA - Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313)](https://www.abcam.cn/ps/products/27/ab27313/Images/ab27313-485042-AntiSerum-Amyloid-PSAP-antibody-14B4-ELISA.jpg)
ELISA - Anti-Serum Amyloid P/SAP antibody [14B4] (ab27313)A direct ELISA was performed using SAP as the coating antigen and ab27313 at a concentration of 0.25ug/mL as the capture antibody
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab27313 被引用在 5 文献中.
- Bergkvist L et al. Serum amyloid P component promotes formation of distinct aggregated lysozyme morphologies and reduces toxicity in Drosophila flies expressing F57I lysozyme. PLoS One 15:e0227227 (2020). PubMed: 31978114
- He X et al. An Improved Method for the Sensitive Detection of Shiga Toxin 2 in Human Serum. Toxins (Basel) 10:N/A (2018). PubMed: 29385045
- Lambert C et al. Phytosterols and Omega 3 Supplementation Exert Novel Regulatory Effects on Metabolic and Inflammatory Pathways: A Proteomic Study. Nutrients 9:N/A (2017). WB ; Human . PubMed: 28608804
- Chen HM et al. Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach. Clin Biochem 44:377-85 (2011). WB . PubMed: 21223953
- Li C et al. Pancreatic cancer serum detection using a lectin/glyco-antibody array method. J Proteome Res 8:483-92 (2009). PubMed: 19072160