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Cardiovascular Blood Acute Phase Reactants

Anti-Serum Amyloid A抗体[115] (ab687)

  • Datasheet
Reviews (6)Q&A (11)References (6)

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Western blot - Anti-Serum Amyloid A antibody [115] (ab687)
  • Western blot - Anti-Serum Amyloid A antibody [115] (ab687)

Key features and details

  • Mouse monoclonal [115] to Serum Amyloid A
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Serum Amyloid A抗体[115]
    参阅全部 Serum Amyloid A 一抗
  • 描述

    小鼠单克隆抗体[115] to Serum Amyloid A
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, Sandwich ELISAmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Serum Amyloid A. Highly purified recombinant human serum amyloid A (MW: 12 kDa).

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 6th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Constituent: PBS
  • Concentration information loading...
  • 纯化说明

    Affinity purified from tissue culture supernatant.
  • 克隆

    单克隆
  • 克隆编号

    115
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Cardiovascular
    • Blood
    • Acute Phase Reactants
    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Lipoproteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab687于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
Use at an assay dependent concentration.

Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA.

Sandwich ELISA
Use at an assay dependent concentration.

sELISA compatible pair - ab18711.

说明
WB
Use at an assay dependent concentration.

Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA.

Sandwich ELISA
Use at an assay dependent concentration.

sELISA compatible pair - ab18711.

靶标

  • 相关性

    Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • 数据库链接

    • Entrez Gene: 694827 Human
    • Omim: 104750 Human
    • SwissProt: P0DJI8 Human
    • SwissProt: P0DJI9 Human
    • 别名

      • Amyloid fibrils antibody
      • SAA 2 antibody
      • Serum amyloid A1 isoform 2 antibody
      • TP53I4 antibody
      • Amyloid fibril protein AA antibody
      • Amyloid protein A antibody
      • Fibrils antibody
      • MGC111216 antibody
      • OC antibody
      • OTTMUSP0000004557 antibody
      • PIG 4 antibody
      • PIG4 antibody
      • SAA 1 antibody
      • SAA antibody
      • SAA_HUMAN antibody
      • SAA1 antibody
      • SAA2 antibody
      • Serum amyloid A 1 antibody
      • serum amyloid A antibody
      • Serum amyloid A protein precursor antibody
      • Serum amyloid A1 isoform 1 antibody
      • Serum amyloid protein A(4-101) antibody
      • Tumor protein p53 inducible protein 4 antibody
      see all

    图片

    • Western blot - Anti-Serum Amyloid A antibody [115] (ab687)
      Western blot - Anti-Serum Amyloid A antibody [115] (ab687)Image courtesy of an anonymous Abreview.
      All lanes : Anti-Serum Amyloid A antibody [115] (ab687) at 1/4000 dilution

      Lanes 1-4 : Acute phase plasma samples
      Lane 5 : Recombinant C-SAA
      Lane 6 : Recombinant A-SAA

      Lysates/proteins at 14 µg per lane.

      Secondary
      All lanes : Goat anti-mouse IgG-HRP at 1/10000 dilution

      Developed using the ECL technique.

      Exposure time: 5 seconds
    • Western blot - Anti-Serum Amyloid A antibody [115] (ab687)
      Western blot - Anti-Serum Amyloid A antibody [115] (ab687)
      Anti-Serum Amyloid A antibody [115] (ab687) at 0.5 µg/ml + Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 1 minute

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab687?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab687 被引用在 6 文献中.

    • Nakayama Y  et al. Spontaneous, Experimentally Induced, and Transmissible AA Amyloidosis in Japanese Quail ( Coturnix japonica). Vet Pathol 54:912-921 (2017). PubMed: 28812532
    • Jahangiri A  et al. Serum amyloid A is found on ApoB-containing lipoproteins in obese humans with diabetes. Obesity (Silver Spring) 21:993-6 (2013). PubMed: 23784902
    • Heo SH  et al. Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy. Proteomics 13:1211-9 (2013). Human . PubMed: 23436750
    • Kramer HB  et al. Elevation of intact and proteolytic fragments of acute phase proteins constitutes the earliest systemic antiviral response in HIV-1 infection. PLoS Pathog 6:e1000893 (2010). WB ; Human . PubMed: 20463814
    • Christenson K  et al. Serum amyloid A inhibits apoptosis of human neutrophils via a P2X7-sensitive pathway independent of formyl peptide receptor-like 1. J Leukoc Biol 83:139-48 (2008). PubMed: 17962368
    • Rossi L  et al. Inflammatory protein profile during systemic high dose interleukin-2 administration. Proteomics 6:709-20 (2006). PubMed: 16342236

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 17 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Serum Amyloid A antibody [115]

    Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana Cell Conditioning 1 buffer
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    OptiView HQ Universal Linker as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 13 2016

    Blocking abreview for Anti-Serum Amyloid A antibody [115]

    Poor
    Abreviews
    Abreviews
    Application
    Blocking
    Sample
    Human Cultured Cells (monocytic cells)
    Specification
    monocytic cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 12 2013

    Western blot abreview for Anti-Serum Amyloid A antibody [115]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Heart, Left Ventricle)
    Loading amount
    80 µg
    Specification
    Heart, Left Ventricle
    Gel Running Conditions
    Reduced Denaturing (10-20% gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dawn Bowles

    Verified customer

    提交于 Dec 28 2012

    Western blot abreview for Anti-Serum Amyloid A antibody [115]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (whole human acute phase plasma)
    Loading amount
    14 µg
    Specification
    whole human acute phase plasma
    Treatment
    heated to 95C in 10-fold excess of reducing SDS sample buffer for 10min
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 21 2011

    Question

    I hope you are well.
    I am writing to follow up on the information I requested regarding immunogen sequencing of two anti-SAA antibodies (ab687 and ab18711) and their compatibility with the equine SAA molecule.
    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you for contacting us.

    I have now received further information from developer lab regarding the products ab687 and ab18711. Regrettably we will not be able to recommend these antibodies, to be used with horse samples. I apologies for any inconvenience.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Oct 17 2012

    Question

    I hope you are well.

    I am writing to follow up on the information I requested regarding immunogen sequencing of two anti-SAA antibodies (ab687 and ab18711) and their compatibility with the equine SAA molecule.



    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 16 2012

    Answer

    Thank you for your email.

    Finally, I have checked the sequence homology between human and horse SAA proteins, the homology is quite low with matching stretches.

    http://www.uniprot.org/blast/uniprot/2012101621EV411P6G?alignment=27


    P0DJI9 is for human SSA protein and F6PJF6 is the ID for horse protein.

    In order to further check whether the antibodies ab687 and ab18711 would be suitable, I have contacted the developer laboratory of these antibodies and asked if they can match the epitope of these antibodies with horse protein sequence. I will send you the details soon.

    The other antibodies we have in catalogue are mostly against the full length human proteins so I am sorry we may not be able to support the horse species cross reactivity. We however have rabbit monoclonal that have >95% immunogen sequence homology with horse so this antibody should cross react with horse.

    https://www.abcam.com/Serum-Amyloid-A-antibody-EPR4134-ab108992.html


    Along with ab108992 you can search an anti SAA antibody which detects N-terminal end of protein, to use these antibodies in sELISA. I am sorry we do not have N-terminal specific products.


    I hope this information will nevertheless helpful. Please do not hesitate to contact me with further inquiries.

    Read More

    Abcam Scientific Support

    回复于 Oct 16 2012

    Question

    I am interested in purchasing two monoclonal anti-serum amyloid A antibodies for the purpose of developing an assay.
    Can you tell me if the following antibodies are compatible pairs? ie. will steric hindrance occur on the target SAA molecule if both antibodies are used in an assay?Ab687
    Ab187313
    Alternatively, can you recommend a known compatible anti-SAA pair?
    I would also like to enquire about long-term availability of your products and up-scaling for manufacturing.
    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2012

    Answer

    Thank you for contacting us.

    We can recommend paring between ab687 and ab18711 instead of ab18713

    https://www.abcam.com/serum-Amyloid-A-antibody-607-ab18711.html

    https://www.abcam.com/Serum-Amyloid-A-antibody-115-ab687.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Oct 10 2012

    Question

    Good morning,
    I wonder how much antibody (ab687) is specific for the gene SAA1,
    since western blot performed with recombinant SAA1, SAA2 and SAA4 has
    been able to identify all. What antibody for western blot assure you
    that is specific for the genes SAA1, SAA2 and SAA4?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 17 2012

    Answer

    Thank you for contacting us. The antibody ab687 is reactive towards the Serum Amyloid A protein and not specific variants.. We do not have any antibodies specific for SAA1,SAA2 or SAA4.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Aug 17 2012

    Question

    Phone call requesting information on if anti-Serum Amyloid A antibodies ab687 and ab81483 are likely to work in native western blotting.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 23 2012

    Answer

    Thank you for contacting us.

    I have looked into what applications anti-Serum Amyloid A antibodies ab687 and ab81483 have been used for in order to ascertain if they are likely to detect the native epitope of Serum Amyloid A in order to perform native western blotting.

    Both ab687 and ab81483 have been used in ELISA where the native epitope is presented. The ELISA carried out with ab687 was performed with the endogenously expressed protein, whilst the ELISA performed with ab81483 used recombinantly expressed protein. Both of these proteins are therefore likely to also be able to detect the protein when performing a native western blot. However, as we have not carried out this experiment I cannot guarantee that it will.

    I also looked into what protocol had been used in performing immunoprecipitation with ab81483, however, the lab were unfortunately able to confirm that they had not tested the antibody in this application and that the informationhad been added to the datasheet in error. The datasheet has now been updated to reflect this.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Mar 23 2012

    Question

    ab1793: IHC-P image legend says "ab1793 staining TNF alpha in mouse liver tissue sections ... An undiluted HRP-conjugated monoclonal to rabbit IgG (1/200) was used as secondary antibody. " But the primary antibody is a mouse monoclonal Ab. will test ab687 in mouse (homology is 74%)

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2011

    Answer

    Thank you for your phone call. 1) Regarding the image for ab1793, this was submitted by a customer as Abreview. For the details of the Abreview please follow this link: https://www.abcam.com/TNF-alpha-antibody-52B83-ab1793.html&tab=abreviews&intabreviewid=17818 It might well be that the monoclonal rabbit IgG secondary antibody is part of the DAKO EnVision+ System, Peroxidase/DAB kit mentioned in the Abreview. But unfortunately, I do not have more information in this regard. ------------------------------------------------------------------------- 2) As for the testing discount for ab687, here is the necessary information: DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab687 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is of help to you. Please let me know if I can assist you further with our products.

    Read More

    Abcam Scientific Support

    回复于 Dec 07 2011

    1-10 of 17 Abreviews or Q&A

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