AB248726

重组Anti-SERPING1抗体[EPR8016] - BSA and Azide free

Anti-SERPING1 antibody [EPR8016] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SERPING1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

C1IN, C1NH, SERPING1, Plasma protease C1 inhibitor, C1 Inh, C1Inh, C1 esterase inhibitor, C1-inhibiting factor, Serpin G1

2 Images

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Western blot - Anti-SERPING1 antibody [EPR8016] - BSA and Azide free (AB248726)

This data was developed using ab134915, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SERPING1 antibody [EPR8016] - BSA and Azide free (ab248726) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 55 kDa

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OI-RD Scanning - Anti-SERPING1 antibody [EPR8016] - BSA and Azide free (AB248726)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SERPING1 antibody [EPR8016] - BSA and Azide free (AB248726)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Unconjugated

Anti-SERPING1 antibody [EPR8016]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8016

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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产品详情

ab248726 is the carrier-free version of ab134915.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SERPING1 also known as C1 inhibitor or C1-inactivator is a protein that plays an important mechanical role in the regulation of the complement system. It is a member of the serpin superfamily and has a molecular mass of approximately 105-110 kDa. This protein is mainly expressed in the liver but also present in other tissues such as monocytes and epithelial cells. As an inhibitor SERPING1 effectively regulates the activity of the complement subcomponents C1r and C1s plasmin and kallikrein by inhibiting their protease activity preventing unwanted activation of the complement cascade.

Biological function summary

The protein acts as a critical control point in the complement and contact activation system. It is part of the C1 complex which includes the proteins C1q C1r and C1s. The regulation extends to pathways that involve the breakdown of plasma proteins and prevention of spontaneous inflammatory reactions. By controlling these pathways the protein prevents excessive inflammation and tissue damage which can occur during immune responses. Its involvement extends to the regulation of the complement system and maintenance of vascular permeability.

Pathways

SERPING1 interacts closely with mechanisms involved in the complement cascade and the kinin-kallikrein pathways. It associates with proteins like MASP-1 and MASP-2 important in the complement and lectin pathways. These pathways are important for innate immunity and influence factors like inflammation and blood pressure. SERPING1 modulates these pathways by inhibiting key enzymes and ensuring controlled responses therefore maintaining systemic balance within the immune system.

The malfunction or deficiency of SERPING1 has direct links to hereditary angioedema a condition marked by severe and rapid swelling episodes. The disorder results from inadequate inhibition of bradykinin emphasizing the protein's role in vascular regulation. Additionally the protein has implications in autoimmune diseases such as systemic lupus erythematosus where complement regulation is disrupted. The interplay between SERPING1 and other proteins such as bradykinin receptor B2 remains significant in understanding disease mechanisms and developing therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

See full target information SERPING1

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重组Anti-SERPING1抗体[EPR8016] - BSA and Azide free