AB281262

重组Anti-SerpinB3/SCCA抗体[EPR22455-5] - BSA and Azide free (Capture)

Anti-SerpinB3/SCCA antibody [EPR22455-5] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SerpinB3/SCCA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

SCCA, SCCA1, SERPINB3, Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1

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Sandwich ELISA - Anti-SerpinB3/SCCA antibody [EPR22455-5] - BSA and Azide free (Capture) (AB281262)

sELISA

Lab

Sandwich ELISA - Anti-SerpinB3/SCCA antibody [EPR22455-5] - BSA and Azide free (Capture) (AB281262)

Sandwich ELISA of ab253702 with the capture antibody ab281262 dilution at 2 µg/mL and detector antibody ab281112 dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22455-5

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab281262 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human SERPINB3. The recommended pair for sandwich ELISA is:
Capture: ab281262, Human SERPINB3 Capture Antibody (unconjugated)
Detector: ab281112, Human SERPINB3 Detector Antibody (unconjugated)
The reference range value is 15.63-4000 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB3 also known as Squamous Cell Carcinoma Antigen (SCCA) is part of the serine protease inhibitor family. This protein has a molecular mass of approximately 45 kDa. It is expressed in various human tissues including skin liver and the respiratory epithelium. SerpinB3 acts by inhibiting cysteine proteinases through binding to them which helps regulate their activity and prevent excessive proteolysis. Such regulatory mechanisms are essential for maintaining cellular homeostasis.

Biological function summary

SerpinB3 plays a role in cellular proliferation differentiation and protection against apoptosis. It is not reported as part of a specific protein complex but it engages in interactions that influence cell signaling pathways. By protecting cells from programmed cell death SerpinB3 can contribute to tissue repair and regeneration. Its expression levels can change significantly during cell stress responses suggesting its involvement in adaptive processes to environmental stimuli.

Pathways

Protein SerpinB3 is involved in inflammatory and immune pathways. It influences the NF-κB signaling pathway which is important for immune response regulation and inflammation. This pathway involves multiple proteins including IκB which acts as an inhibitor and other cytokines and transcription factors that facilitate signal transduction in response to stress. SerpinB3’s modulation of these pathways reflects how it affects both innate and adaptive immune responses.

SerpinB3 has been associated with the progression of squamous cell carcinoma and liver fibrosis. In squamous cell carcinoma its interaction with proteases like cathepsin contributes to cancer cell survival and metastasis. For liver fibrosis SerpinB3’s inhibition of apoptosis in hepatocytes may lead to excessive tissue scarring. These connections illustrate its potential as a biomarker for diagnosis and as a target for therapeutic intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).

See full target information SERPINB3

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SerpinB3/SCCA抗体[EPR22455-5] - BSA and Azide free (Capture)

Anti-SerpinB3/SCCA antibody [EPR22455-5] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-SerpinB3/SCCA antibody [EPR22455-5] - BSA and Azide free (Capture) (AB281262)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原