Anti-SerpinB2/PAI-2抗体(ab47742)
Key features and details
- Rabbit polyclonal to SerpinB2/PAI-2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SerpinB2/PAI-2抗体
参阅全部 SerpinB2/PAI-2 一抗 -
描述
兔多克隆抗体to SerpinB2/PAI-2 -
宿主
Rabbit -
特异性
This antibody is specific for SerpinB2/PAI-2 (but not the shorter form).
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SerpinB2/PAI-2 (N terminal).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47742于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| WB | (1) |
| 说明 |
|---|
应用说明
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 46 kDa. Dilution optimised using Chromogenic detection.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 46 kDa. Dilution optimised using Chromogenic detection.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. -
序列相似性
Belongs to the serpin family. Ov-serpin subfamily. -
翻译后修饰
The signal sequence is not cleaved. -
细胞定位
Cytoplasm. Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 5055 Human
- Omim: 173390 Human
- SwissProt: P05120 Human
- Unigene: 594481 Human
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别名
- HsT1201 antibody
- Monocyte Arg serpin antibody
- Monocyte Arg-serpin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody (ab47742)Ab47742 staining human normal temporal lobe. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-SerpinB2/PAI-2 antibody (ab47742)Anti-SerpinB2/PAI-2 antibody (ab47742) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteSerpinB2/PAI-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab47742 被引用在 10 文献中.
- Park SR et al. Effects of smoking on the tissue regeneration-associated functions of human endometrial stem cells via a novel target gene SERPINB2. Stem Cell Res Ther 13:404 (2022). PubMed: 35932085
- Vogel-González M et al. Zinc Favors Triple-Negative Breast Cancer's Microenvironment Modulation and Cell Plasticity. Int J Mol Sci 22:N/A (2021). PubMed: 34502091
- Park SR et al. Sonic Hedgehog, a Novel Endogenous Damage Signal, Activates Multiple Beneficial Functions of Human Endometrial Stem Cells. Mol Ther 28:452-465 (2020). PubMed: 31866117
- Lee NH et al. SERPINB2 Is a Novel Indicator of Cancer Stem Cell Tumorigenicity in Multiple Cancer Types. Cancers (Basel) 11:N/A (2019). PubMed: 30965654
- Zhang XM et al. SERPINB2 overexpression inhibited cell proliferation, invasion and migration, led to G2/M arrest, and increased radiosensitivity in nasopharyngeal carcinoma cells. J Radiat Res 60:318-327 (2019). PubMed: 30864656
- Cho A et al. An Endogenous Anti-aging Factor, Sonic Hedgehog, Suppresses Endometrial Stem Cell Aging through SERPINB2. Mol Ther 27:1286-1298 (2019). PubMed: 31080015
- Lee NH et al. SERPINB2 is a novel indicator of stem cell toxicity. Cell Death Dis 9:724 (2018). PubMed: 29925837
- Mertens TCJ et al. Cigarette smoke differentially affects IL-13-induced gene expression in human airway epithelial cells. Physiol Rep 5:N/A (2017). WB . PubMed: 28701525
- Peng H et al. microRNA-31/factor-inhibiting hypoxia-inducible factor 1 nexus regulates keratinocyte differentiation. Proc Natl Acad Sci U S A 109:14030-4 (2012). WB, IHC-P ; Human . PubMed: 22891326
- Angelucci S et al. Proteome analysis of human Wharton's jelly cells during in vitro expansion. Proteome Sci 8:18 (2010). WB ; Human . PubMed: 20346146