AB267463

重组Anti-SerpinB2/PAI-2抗体[EPR22924-56]

Anti-SerpinB2/PAI-2 antibody [EPR22924-56]

BOND RX™ Validated
RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal SerpinB2/PAI-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

查看别名

PAI2, PLANH2, SERPINB2, Plasminogen activator inhibitor 2, PAI-2, Monocyte Arg-serpin, Placental plasminogen activator inhibitor, Serpin B2, Urokinase inhibitor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skin. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with ematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Unknown

Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (AB267463)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 : 8 seconds; Lane 4 : 10 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28427146).

Negative on HeLa : (PMID : 22334683, 12944478).

All lanes:

Western blot - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] (ab267463) at 1/1000 dilution

Lane 1:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human placenta lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-SerpinB2/PAI-2 antibody [EPR22924-56] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22924-56

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB2 also known as PAI-2 or plasminogen activator inhibitor type 2 is a serine protease inhibitor with a molecular mass of around 60 kDa. This protein localizes mostly in the cytoplasm and occasionally gets secreted outside the cell. SerpinB2 expresses in a variety of cells including monocytes macrophages and certain epithelial cells. Its primary function involves inhibiting the urokinase plasminogen activator important for processes like fibrinolysis and tissue remodeling.

Biological function summary

SerpinB2 acts as a protector against excessive proteolysis by regulating extracellular matrix hydrolysis. While not permanently part of a complex SerpinB2 associates transiently with target proteases to modulate their activities. This regulation plays a role in cell migration and inflammation control impacting wound healing and immune responses.

Pathways

SerpinB2 integrates into the plasminogen activation system which is important for fibrinolysis and maintaining hemostatic balance. It works closely with other serpins and proteases such as PAI-1 and urokinase-type plasminogen activator (uPA) to control the conversion of plasminogen to plasmin. This balance impacts tissue repair and may influence invasive cell behavior through the regulation of pericellular proteolysis.

SerpinB2's alteration has been linked to the development of atherosclerosis and cancer. Research shows that SerpinB2 levels can influence the inflammatory environment possibly driving atherogenic plaque development. Similarly changes in SerpinB2 expression and activity relate to cancer metastasis potentially by affecting the balance of matrix degradation and rebuilding alongside proteins like PAI-1 and uPA to modulate tumor invasiveness and growth.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

See full target information SERPINB2

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiothoracic surgery 19:141 PubMed38504347

2024

Smoking behavior associated upregulation of SERPINB12 promotes proliferation and metastasis via activating WNT signaling in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Zhen Zheng,Xiang Miao,Jing Chang,Hai Zhou,Jing-Jian Zhang,Hui-Min Mo,Qin Jia

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SerpinB2/PAI-2抗体[EPR22924-56]