Anti-SERPINB1/PI2抗体(ab47731)
Key features and details
- Rabbit polyclonal to SERPINB1/PI2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SERPINB1/PI2抗体
参阅全部 SERPINB1/PI2 一抗 -
描述
兔多克隆抗体to SERPINB1/PI2 -
宿主
Rabbit -
特异性
This antibody is specific for SERPINB1/PI2.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SERPINB1/PI2 (N terminal).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. -
序列相似性
Belongs to the serpin family. Ov-serpin subfamily. -
结构域
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P30740 Human
- Unigene: 381167 Human
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别名
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
图片
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All lanes : Anti-SERPINB1/PI2 antibody (ab47731) at 1/1000 dilution
All lanes : MH-S cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size: 43 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible degradation product) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody (ab47731)ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab47731 被引用在 5 文献中.
- Sommeregger W et al. Proteomic differences in recombinant CHO cells producing two similar antibody fragments. Biotechnol Bioeng 113:1902-12 (2016). WB . PubMed: 26913574
- van Kempen PM et al. Oropharyngeal squamous cell carcinomas differentially express granzyme inhibitors. Cancer Immunol Immunother 65:575-85 (2016). PubMed: 26993499
- Vermeulen JF et al. Pediatric Primitive Neuroectodermal Tumors of the Central Nervous System Differentially Express Granzyme Inhibitors. PLoS One 11:e0151465 (2016). PubMed: 26963506
- Uchiyama K et al. Serpin B1 protects colonic epithelial cell via blockage of neutrophil elastase activity and its expression is enhanced in patients with ulcerative colitis. Am J Physiol Gastrointest Liver Physiol 302:G1163-70 (2012). WB, IHC-P ; Human . PubMed: 22421620
- Tseng MY et al. Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. Oral Oncol 45:771-6 (2009). WB, IHC-P ; Human . PubMed: 19213596