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重组RabMAb

重组Anti-Serotonin transporter抗体[EPR23530-3] - BSA and Azide free (ab275094)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
  • Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
  • Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
  • Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
  • Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
  • Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23530-3] to Serotonin transporter - BSA and Azide free
  • Suitable for: IHC-Fr, IP, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Serotonin transporter抗体[EPR23530-3] - BSA and Azide free
    参阅全部 Serotonin transporter 一抗

  • 描述

    兔单克隆抗体[EPR23530-3] to Serotonin transporter - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Fr, IP, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or WB

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse and rat cerebrum tissue. IHC-Fr: Mouse cerebrum tissue. IP: Mouse and rat brain tissue lysate.

  • 常规说明

    ab275094 is the carrier-free version of ab254358.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab275094于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.


Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
IHC-Fr
Use at an assay dependent concentration.


Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or WB.

靶标

  • 功能

    Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

  • 组织特异性

    Expressed in platelets (at protein level).

  • 序列相似性

    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

  • 翻译后修饰

    Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
    Phosphorylated at Ser-611, Thr-613 and Thr-616.

  • 细胞定位

    Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 5 HTT antibody
      • 5 HTTLPR antibody
      • 5 hydroxytryptamine (serotonin) transporter antibody
      • 5 hydroxytryptamine transporter antibody
      • 5HT transporter antibody
      • 5HTT antibody
      • hSERT antibody
      • HTT antibody
      • Na+/Cl- dependent serotonin transporter antibody
      • OCD1 antibody
      • SC6A4_HUMAN antibody
      • Serotonin transporter 1 antibody
      • SERT antibody
      • SERT1 antibody
      • Slc6a4 antibody
      • Sodium dependent serotonin transporter antibody
      • Sodium-dependent serotonin transporter antibody
      • Solute carrier family 6 (neurotransmitter transporter) member 4 antibody
      • solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 antibody
      • Solute carrier family 6 member 4 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

      This data was developed using ab254358, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID 23127756). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

      This data was developed using ab254358, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Serotonin transporter with ab254358 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on synapses of neurons in mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

      This data was developed using ab254358, the same antibody clone in a different buffer formulation.

      Serotonin transporter was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab254358 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254358 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse brain tissue lysate 10 µg

      Lane 2: ab254358 IP in mouse brain tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254358 in mouse brain tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

      This data was developed using ab254358, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets and negative staining on hepatocytes of mouse liver (PMID: 7869097). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

      This data was developed using ab254358, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID 23127756). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

      This data was developed using ab254358, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Serotonin transporter with ab254358 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

      Negative control: No staining on mouse liver (PMID: 9037532) is observed. 

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

      This data was developed using ab254358, the same antibody clone in a different buffer formulation.

      Serotonin transporter was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab254358 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254358 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Rat brain tissue lysate 10 µg

      Lane 2: ab254358 IP in rat brain tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254358 in rat brain tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 33 seconds.

       

    • Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
      Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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