Anti-Serotonin transporter抗体(ab272912)
Key features and details
- Rabbit polyclonal to Serotonin transporter
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Serotonin transporter抗体
参阅全部 Serotonin transporter 一抗 -
描述
兔多克隆抗体to Serotonin transporter -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat
预测可用于: Human
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免疫原
Synthetic peptide within Mouse Serotonin transporter. The exact sequence is proprietary. Carrier-protein conjugated. Center region.
Database link: Q60857 -
阳性对照
- WB: Rat intestine tissue extract. ICC/IF: DIV9 rat E18 primary cortical neuron cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272912于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. -
组织特异性
Expressed in platelets (at protein level). -
序列相似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily. -
翻译后修饰
Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylated at Ser-611, Thr-613 and Thr-616. -
细胞定位
Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. - Information by UniProt
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数据库链接
- Entrez Gene: 6532 Human
- Entrez Gene: 25553 Rat
- Omim: 182138 Human
- SwissProt: P31645 Human
- SwissProt: P31652 Rat
- Unigene: 134662 Human
- Unigene: 1663 Rat
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别名
- 5 HTT antibody
- 5 HTTLPR antibody
- 5 hydroxytryptamine (serotonin) transporter antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Serotonin transporter antibody (ab272912)
4% PFA-fixed DIV9 rat E18 primary cortical neuron cells stained for Serotonin transporter using ab272912 at 1/500 dilution in ICC/IF.
Red: Beta tubulin.
Blue: DAPI.
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Western blot - Anti-Serotonin transporter antibody (ab272912)Anti-Serotonin transporter antibody (ab272912) at 1/500 dilution + Rat intestine tissue lysate at 50 µg7.5% SDS-PAGE.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272912 被引用在 1 文献中.
- Zhang B et al. Environmentally-relevant concentrations of the antipsychotic drugs sulpiride and clozapine induce abnormal dopamine and serotonin signaling in zebrafish brain. Sci Rep 12:17973 (2022). PubMed: 36289270