重组Anti-Serotonin抗体[EPR24431-201]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Serotonin with ab315150 at 1/4000 (0.132 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on enterochromaffin cells of human colon (PMID : 17195902).The section was incubated with ab315150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Serotonin with ab315150 at 1/4000 (0.132 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on enterochromaffin cells of mouse colon (PMID : 18192499).The section was incubated with ab315150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Serotonin with ab315150 at 1/4000 (0.132 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on enterochromaffin cells of rat colon (PMID : 27840918).The section was incubated with ab315150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling Eph receptor B2 with ab252935 at 1/500 dilution (B), GSDMC2 + GSDMC3 with ab229896 at 1/500 dilution (C) and Serotonin with ab315150 at 1/500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Eph receptor B2 (green; Opal™520), anti-GSDMC2/3 (magenta; Opal™690) and anti-Serotonin (gray; Opal™570) on mouse stomach.

Panel B : anti-Eph receptor B2 staining stem cells in mouse stomach.

Panel C : anti-GSDMC2/3 staining epithelium in mouse stomach.

Panel D : anti-Serotonin staining enterochromaffin cells in mouse stomach.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab252935, ab229896 and ab315150 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse small intestine tissue staining CaMKII beta with ab314897 at a 1/5000 dilution, ab315150 anti-Serotonin used at 1/5000 dilution and ab238123 anti-MPTX used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CaMKII beta (green; Opal^™690), anti-Serotonin (magenta; Opal^™520) and anti-MPTX (grey; Opal^™570) on mouse small intestine.
Panel B : anti-CaMKII beta staining myenteric nerve plexus and endocrine cells in mouse small intestine.
Panel C : anti-Serotonin staining enterochromaffin cells in mouse small intestine.
Panel D : anti-MPTX staining membrane of Paneth cells in mouse small intestine.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab314897, ab315150 and ab238123 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse stomach tissue staining CaMKII beta with ab314897 at 1/5000 dilution, ab315150 anti-Serotonin used at 1/5000 dilution and ab209790 anti-Ghrelin used at a 1/40000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CaMKII beta (magenta; Opal^™690), anti-Serotonin (green; Opal^™520) and anti-Ghrelin (gray; Opal^™570) on mouse stomach.
Panel B : anti-CaMKII beta staining endocrine cells in mouse stomach.
Panel C : anti-Serotonin staining enterochromaffin cells in mouse stomach.
Panel D : anti-Ghrelin staining ghrelin in mouse stomach.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314897, ab315150 and ab209790 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse stomach tissue staining CaMKII beta with ab314897 at 1/5000 dilution, ab315150 anti-Serotonin used at 1/5000 dilution and ab287960 anti-Aurora B used at a 1/300 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CaMKII beta (magenta; Opal^™690), anti-Serotonin (green; Opal^™520) and anti-Aurora B (gray; Opal^™570) on mouse stomach.
Panel B : anti-CaMKII beta staining endocrine cells in mouse stomach.
Panel C : anti-Serotonin staining enterochromaffin cells in mouse stomach.
Panel D : anti-Aurora B staining proliferating cells in mouse stomach.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314897, ab315150 and ab287960 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Indirect ELISA analysis of ab315150 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Serotonin cross-linked to BSA.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Serotonin antibody [EPR24431-201] (AB315150)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In lane 1, anti-BSA could not detect the total level of cross-linked BSA due to the blocking of epitopes by serotonin.

In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Serotonin antibody [EPR24431-201] (ab315150) at 1/1000 dilution

Lane 1:

Serotonin cross-linked to BSA at 20 ng

Lane 2:

BSA

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 69 kDa

false

Exposure time: 70s