Anti-SERCA2 ATPase抗体(ab183531)
Key features and details
- Goat polyclonal to SERCA2 ATPase
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SERCA2 ATPase抗体
参阅全部 SERCA2 ATPase 一抗 -
描述
山羊多克隆抗体to SERCA2 ATPase -
宿主
Goat -
特异性
ab183531 is expected to recognize all 5 reported isoforms. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Cat, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human SERCA2 ATPase aa 650-750 (internal sequence).
Database link: P16615 -
阳性对照
- WB: Human, mouse and rat heart lysates. ICC/IF: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab183531 was purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183531于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 115 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 115 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. -
组织特异性
Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. -
疾病相关
Defects in ATP2A2 are a cause of acrokeratosis verruciformis (AKV) [MIM:101900]; also known as Hopf disease. AKV is a localized disorder of keratinization, which is inherited as an autosomal dominant trait. Its onset is early in life with multiple flat-topped, flesh-colored papules on the hands and feet, punctate keratoses on the palms and soles, with varying degrees of nail involvement. The histopathology shows a distinctive pattern of epidermal features with hyperkeratosis, hypergranulosis, and acanthosis together with papillomatosis. These changes are frequently associated with circumscribed elevations of the epidermis that are said to resemble church spires. There are no features of dyskeratosis or acantholysis, the typical findings in lesions of Darier disease.
Defects in ATP2A2 are the cause of Darier disease (DD) [MIM:124200]; also known as Darier-White disease (DAR). DD is an autosomal dominantly inherited skin disorder characterized by loss of adhesion between epidermal cells (acantholysis) and abnormal keratinization. Patients with mild disease may have no more than a few scattered keratotic papules or subtle nail changes, whereas those with severe disease are handicapped by widespread malodorous keratotic plaques. In a few families, neuropsychiatric abnormalities such as mild mental retardation, schizophrenia, bipolar disorder and epilepsy have been reported. Stress, UV exposure, heat, sweat, friction, and oral contraception exacerbate disease symptoms. Prevalence has been estimated at 1 in 50000. Clinical variants of DD include hypertrophic, vesicobullous, hypopigmented, cornifying, zosteriform or linear, acute and comedonal subtypes. Comedonal Darier disease (CDD) is characterized by the coexistence of acne-like comedonal lesions with typical Darier hyperkeratotic papules on light-exposed areas. At histopathologic level, CDD differs from classic DD in the prominent follicular involvement and the presence of greatly elongated dermal villi. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
翻译后修饰
Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity. -
细胞定位
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 488 Human
- Entrez Gene: 11938 Mouse
- Entrez Gene: 396875 Pig
- Entrez Gene: 100038308 Rabbit
- Entrez Gene: 29693 Rat
- Omim: 108740 Human
- SwissProt: P16615 Human
- SwissProt: O55143 Mouse
see all -
别名
- AT2A2_HUMAN antibody
- Atp2a2 antibody
- ATP2B antibody
see all
图片
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All lanes : Anti-SERCA2 ATPase antibody (ab183531) at 0.1 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Mouse heart lysate (in RIPA buffer)
Lane 3 : Rat heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 115 kDa -
mmunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab183531 被引用在 1 文献中.
- Agarwal A et al. Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells. Microbiol Spectr 10:e0083022 (2022). PubMed: 35604158