重组Anti-SERCA1 ATPase抗体[VE121G9] - BSA and Azide free (ab255956)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [VE121G9] to SERCA1 ATPase - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-SERCA1 ATPase抗体[VE121G9] - BSA and Azide free
参阅全部 SERCA1 ATPase 一抗 -
描述
小鼠单克隆抗体[VE121G9] to SERCA1 ATPase - BSA and Azide free -
宿主
Mouse -
特异性
Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase.
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经测试应用
适用于: WB, IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human skeletal muscle tissue lysate. IHC-P: Human, mouse and rat skeletal muscle tissue.
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常规说明
ab255956 is the carrier-free version of ab2819.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
VE121G9 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab255956于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa).
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa). |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
组织特异性
Skeletal muscle, fast twitch muscle (type II) fibers. -
疾病相关
Brody myopathy -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
发展阶段
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
细胞定位
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Omim: 108730 Human
- SwissProt: O14983 Human
- SwissProt: Q8R429 Mouse
- Unigene: 657344 Human
- Unigene: 35134 Mouse
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别名
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
图片
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This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide (ab2819).
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA1 ATPase with ab255956 at 1/200 dilution, followed by ready to use secondary. Cytoplasmic staining on human skeletal muscle tissue is observed. The section was incubated with ab2819 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary. -
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, (ab2819).
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab255956 at 1/200 dilution, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2819 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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