重组Anti-SERCA1 ATPase抗体[IIH11] (ab2818)
![Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.cn/ps/products/2/ab2818/Images/ab2818-367710-AntiSERCA1-ATPase-antibody-IIH11-western-blot-skeletal-muscle-mouse-rat.jpg "Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [IIH11] to SERCA1 ATPase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-SERCA1 ATPase抗体[IIH11]
参阅全部 SERCA1 ATPase 一抗 -
描述
小鼠单克隆抗体[IIH11] to SERCA1 ATPase -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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表位
This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum. -
阳性对照
- WB: Mouse and rat skeletal muscle tissue lysate. IHC-P: Mouse and rat skeletal muscle tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
IIH11 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.466 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
|
| IHC-P |
Use a concentration of 0.177 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.466 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
|
IHC-P
Use a concentration of 0.177 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
组织特异性
Skeletal muscle, fast twitch muscle (type II) fibers. -
疾病相关
Brody myopathy -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
发展阶段
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
细胞定位
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11937 Mouse
- Entrez Gene: 116601 Rat
- SwissProt: Q8R429 Mouse
- SwissProt: Q64578 Rat
- Unigene: 35134 Mouse
- Unigene: 217139 Rat
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别名
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
图片
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Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)All lanes : Anti-SERCA1 ATPase antibody [IIH11] (ab2818) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 48 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.cn/ps/products/2/ab2818/Images/ab2818-367709-AntiSERCA1-ATPase-antibody-IIH11-immunohistochemistry-skeletal-muscle-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.177µg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.cn/ps/products/2/ab2818/Images/ab2818-367707-AntiSERCA1-ATPase-antibody-IIH11-immunohistochemistry-skeletal-muscle-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.
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![Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.cn/ps/products/2/ab2818/Images/ab2818-10-benefits-of-recombinant-antibodies.png)
Anti-SERCA1 ATPase antibody [IIH11] (ab2818)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab2818 被引用在 10 文献中.
- Hofhuis J et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). PubMed: 28104817
- Murphy S et al. Concurrent Label-Free Mass Spectrometric Analysis of Dystrophin Isoform Dp427 and the Myofibrosis Marker Collagen in Crude Extracts from mdx-4cv Skeletal Muscles. Proteomes 3:298-327 (2015). PubMed: 28248273
- Murphy S et al. Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy. Biology (Basel) 4:397-423 (2015). PubMed: 26067837
- Funai K et al. Muscle lipogenesis balances insulin sensitivity and strength through calcium signaling. J Clin Invest 123:1229-40 (2013). PubMed: 23376793
- Hernández A et al. Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle. J Physiol 590:3575-83 (2012). PubMed: 22687611
- Abcejo AJ et al. Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle. PLoS One 7:e44343 (2012). PubMed: 22952960
- Yamada T et al. Impaired mitochondrial respiration and decreased fatigue resistance followed by severe muscle weakness in skeletal muscle of mitochondrial DNA mutator mice. J Physiol 590:6187-97 (2012). PubMed: 22988144
- Harris RL et al. Tail muscle parvalbumin content is decreased in chronic sacral spinal cord injured rats with spasticity. Exp Physiol 96:1311-20 (2011). PubMed: 21930674
- Bruton JD et al. Increased fatigue resistance linked to Ca2+-stimulated mitochondrial biogenesis in muscle fibres of cold-acclimated mice. J Physiol 588:4275-88 (2010). PubMed: 20837639
- Eizema K et al. Differential expression of calcineurin and SR Ca2+ handling proteins in equine muscle fibers during early postnatal growth. J Histochem Cytochem 55:247-54 (2007). PubMed: 17101725