AB154112

Anti-Septin 8抗体

Name: Septin 8抗体 (ab154112)| Abcam中文官网
Brand: Abcam Limited
SKU: AB154112
Price: 4606 CNY
Availability: InStock

Anti-Septin 8 antibody

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(1 Publication)

Rabbit Polyclonal Septin 8 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN8 aa 150 to C-terminus.

查看别名

KIAA0202, SEPT8, SEPTIN8, Septin-8

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling Septin 8 with ab154112 at 1/200 dilution (green).
Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling SEPT8 protein using ab154112 at a 1/500 dilution (Green).
beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

Unknown

Western blot - Anti-Septin 8 antibody (AB154112)

12% SDS PAGE

All lanes:

Western blot - Anti-Septin 8 antibody (ab154112) at 1/2000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 56 kDa

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Supplier Data

Western blot - Anti-Septin 8 antibody (AB154112)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Septin 8 antibody (ab154112) at 1/4000 dilution

Lane 1:

Mouse brain tissue extracts at 10 µg

Lane 2:

Rat brain tissue extracts at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human SEPTIN8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92599

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Septin 8 often referred to as SEPT8 belongs to the septin family of GTPase proteins. It participates in filament formation by polymerizing with other septins. This protein has a molecular weight of around 52 kDa. SEPT8 is expressed in various tissues with significant presence in the brain and heart. Its function depends on cell type and developmental stage exhibiting dynamic patterns of expression which make it integral to cellular processes.

Biological function summary

The protein is involved in maintaining cell structure and polarity. SEPT8 is part of larger septin complexes necessary for correct cytokinesis. It regulates cell division by contributing to the formation of cleavage furrow during mitosis. SEPT8 also influences membrane dynamics and vesicle trafficking showcasing a role in transport within cells.

Pathways

Septin 8 contributes to cytoskeleton organization and vesicle trafficking pathways. It associates with proteins such as actin and myosin playing roles in cell shape changes and intracellular transport. SEPT8 functions alongside other septins in the septin ring structures which are essential for several processes including cell cycle progression and vesicle sorting.

Septin 8 has been linked to neurodegenerative conditions and cancer. Abnormal SEPT8 expression or mutations might lead to Alzheimer's disease due to its impact on neuronal transport systems. SEPT8 is also observed in tumorigenesis interfering with normal cell cycle control. In cancer its interaction with other septins could disrupt normal cell proliferation suggesting it as a potential therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (PubMed : 15116257). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).. Isoform 4. Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP.

See full target information SEPTIN8

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:106-122.e9 PubMed34875212

2021

SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity.

Applications

Unspecified application

Species

Unspecified reactive species

Daoud Sheban,Tom Shani,Roey Maor,Alejandro Aguilera-Castrejon,Nofar Mor,Bernardo Oldak,Merav D Shmueli,Avital Eisenberg-Lerner,Jonathan Bayerl,Jakob Hebert,Sergey Viukov,Guoyun Chen,Assaf Kacen,Vladislav Krupalnik,Valeriya Chugaeva,Shadi Tarazi,Alejandra Rodríguez-delaRosa,Mirie Zerbib,Adi Ulman,Solaiman Masarwi,Meital Kupervaser,Yishai Levin,Efrat Shema,Yael David,Noa Novershtern,Jacob H Hanna,Yifat Merbl

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Septin 8抗体

Anti-Septin 8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Western blot - Anti-Septin 8 antibody (AB154112)

Western blot - Anti-Septin 8 antibody (AB154112)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (1)

Abcam Product Promise

Anti-Septin 8抗体

Anti-Septin 8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Western blot - Anti-Septin 8 antibody (AB154112)

Western blot - Anti-Septin 8 antibody (AB154112)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

替代产品

Primary Antibodies

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (1)

Abcam Product Promise