重组Anti-Septin 2抗体[EPR12123] (ab179436)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12123] to Septin 2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Septin 2抗体[EPR12123]
参阅全部 Septin 2 一抗 -
描述
兔单克隆抗体[EPR12123] to Septin 2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Xenopus laevis -
免疫原
Synthetic peptide within Human Septin 2 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15019 -
阳性对照
- Human fetal brain, U87-MG, Jurkat and MCF-7 lysates. Human hepatocellular carcinoma and pancreas tissues. MCF-7 and Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12123 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179436于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
靶标
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功能
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri. -
组织特异性
Widely expressed. Up-regulated in liver cancer. -
序列相似性
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
Contains 1 septin-type G (guanine nucleotide-binding) domain. -
细胞定位
Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Midbody. Cytoplasm, cell cortex. Cell projection, cilium membrane. In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus. Localizes at the base of the cilia near the morphological distinction between the cilia and plasma membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 4735 Human
- Entrez Gene: 18000 Mouse
- Entrez Gene: 117515 Rat
- Entrez Gene: 398203 Xenopus laevis
- Entrez Gene: 494757 Xenopus laevis
- Omim: 601506 Human
- SwissProt: Q15019 Human
- SwissProt: P42208 Mouse
see all -
别名
- DIFF6 antibody
- hNEDD5 antibody
- KIAA0158 antibody
see all
图片
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All lanes : Anti-Septin 2 antibody [EPR12123] (ab179436) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : U87-MG lysate
Lane 3 : Jurkat lysate
Lane 4 : MCF-7 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Septin 2 with ab179436 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Septin 2 with ab179436 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of MCF-7 cells labeling Septin 2 with ab179436 at 1/50 dilution (red). DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized Jurkat cellslabeling Septin 2 with ab179436 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).
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Western blot analysis on immunoprecipitation pellet from (1) MCF-7 cell lysate or (2) 1XPBS (negative control) using ab179436 at a 1/10 dilution for IP, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab179436 被引用在 4 文献中.
- Kim KK et al. Development of Potent Forchlorfenuron Analogs and Their Cytotoxic Effect in Cancer Cell Lines. Sci Rep 10:3241 (2020). PubMed: 32094384
- He H et al. Expression of septin 2 and association with clinicopathological parameters in colorectal cancer. Oncol Lett 18:2376-2383 (2019). PubMed: 31402940
- Xu C et al. Coupling function of cyclin-dependent kinase 2 and Septin2 in the promotion of hepatocellular carcinoma. Cancer Sci 110:540-549 (2019). PubMed: 30444001
- Danson CM et al. Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin. J Cell Sci 131:N/A (2018). PubMed: 30072438