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AB249486

重组Anti-SEPT10抗体[EPR11141(B)] - BSA and Azide free

Anti-SEPT10 antibody [EPR11141(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal SEPT10 antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

SEPT10, SEPTIN10, Septin-10

1 Images
Western blot - Anti-SEPT10 antibody [EPR11141(B)] - BSA and Azide free (AB249486)
  • WB

Unknown

Western blot - Anti-SEPT10 antibody [EPR11141(B)] - BSA and Azide free (AB249486)

This data was developed using ab169533, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SEPT10 antibody [EPR11141(B)] (<a href='/products/primary-antibodies/sept10-antibody-epr11141b-ab169533'>ab169533</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

PC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11141(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

ab249486 is the carrier-free version of ab169533.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SEPT10 also known as Septin-10 is a member of the septin family characterized by its GTP-binding capability. This protein has a molecular weight of approximately 50 kDa. SEPT10 plays a significant role in cellular processes involving cytoskeletal organization. It is commonly expressed in brain lung and spleen tissues. Septins like SEPT10 form filament structures through hetero-oligomerization allowing for compartmentalization and barrier formation within cells.
Biological function summary

SEPT10 participates in cellular division and morphogenesis. It forms part of larger septin complexes which include other septin proteins acting as a scaffold for recruiting and localizing proteins essential for various biological processes. In addition SEPT10 helps in maintaining cell integrity and shape by regulating the organization of actin and microtubules contributing to centrosome organization during cell division.

Pathways

SEPT10 integrates into pathways related to cell cycle regulation and cytoskeleton organization. In particular it associates with the Rho-signaling pathway influencing cell polarity and shape. In these pathways SEPT10 interacts with proteins like Cdc42 and RhoA contributing to the dynamic remodeling of the cytoskeleton and influencing cellular responses to external stimuli.

SEPT10 has connections to cancer and neurodegenerative conditions. Alterations in SEPT10 expression levels or mutations can disrupt normal cell division influencing tumorigenesis in certain cancers. For example changes in SEPT10 have been observed in glioma a type of brain cancer. Additionally its interaction with amyloid-beta precursor protein links it to Alzheimer's disease where impaired neurogenesis and protein aggregation are prevalent. Understanding these interactions may provide deeper insights into therapeutic targets for these diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential).
See full target information SEPTIN10
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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