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重组RabMAb

重组Anti-SEP15抗体[NCIR128A] (ab124840)

Reviews (1) Submit a question References (9)
Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
  • Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
  • Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
  • Anti-SEP15 antibody [NCIR128A] (ab124840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [NCIR128A] to SEP15
  • Suitable for: WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-SEP15抗体[NCIR128A]
    参阅全部 SEP15 一抗

  • 描述

    兔单克隆抗体[NCIR128A] to SEP15

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Rat, Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide corresponding to Mouse SEP15.

  • 阳性对照

    • WB: Rat liver, fetal liver, human testis, mouse testis, rat testis and LnCaP lysates; 293T transfected with N-terminal tagged human SEP15 expression vectors whole cell lysate; Human SEP15 full length recombinant protein.

  • 常规说明

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    NCIR128A

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124840于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.

C-terminal tagged SEP15 expression vectors might be not recognized by ab124840.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.

C-terminal tagged SEP15 expression vectors might be not recognized by ab124840.

靶标

图片

  • Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
    Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
    All lanes : Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution

    Lane 1 : 293T transfected with C-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg
    Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), whole cell lysate at 15 µg
    Lane 3 : 293T transfected with N-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg
    Lane 4 : Human SEP15 full length recombinant protein

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST

    C-terminal tagged SEP15 expression vectors might be not recognized by ab124840.

  • Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
    Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
    Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (purified) + Human testis lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 18 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
    Western blot - Anti-SEP15 antibody [NCIR128A] (ab124840)
    All lanes : Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (unpurified)

    Lane 1 : Rat liver lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : LnCaP lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 18 kDa

  • Anti-SEP15 antibody [NCIR128A] (ab124840)
    Anti-SEP15 antibody [NCIR128A] (ab124840)

文献 (9)

发表研究结果有使用 ab124840？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab124840 被引用在 9 文献中.

  • Ekyalongo RC  et al. SELENOF Controls Proliferation and Cell Death in Breast-Derived Immortalized and Cancer Cells. Cancers (Basel) 15:N/A (2023). PubMed: 37509331
  • Mideksa YG  et al. A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines. J Biol Chem 298:102677 (2022). PubMed: 36336075
  • Sonet J  et al. Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines. Int J Mol Sci 22:N/A (2021). PubMed: 34298926
  • Zheng X  et al. Selenoprotein F knockout leads to glucose and lipid metabolism disorders in mice. J Biol Inorg Chem 25:1009-1022 (2020). PubMed: 32995962
  • Ekoue DN  et al. Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer. Prostate 78:279-288 (2018). PubMed: 29314169
  • Touat-Hamici Z  et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . PubMed: 29660373
  • Yim SH  et al. Role of Selenof as a Gatekeeper of Secreted Disulfide-Rich Glycoproteins. Cell Rep 23:1387-1398 (2018). PubMed: 29719252
  • Tsuji PA  et al. The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways. PLoS One 10:e0124487 (2015). Human . PubMed: 25886253
  • Howard MT  et al. Translational redefinition of UGA codons is regulated by selenium availability. J Biol Chem 288:19401-13 (2013). WB ; Mouse . PubMed: 23696641

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Western blot abreview for Anti-SEP15 antibody [NCIR128A]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole body)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
whole body
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Apr 23 2018

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