Anti-SENP2抗体[OTI1H2] (ab131637)
![Western blot - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146392-anti-senp2-antibody-oti1h2-western-blot.jpg "Western blot - Anti-SENP2 antibody [OTI1H2] (ab131637)")
Key features and details
- Mouse monoclonal [OTI1H2] to SENP2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-SENP2抗体[OTI1H2]
参阅全部 SENP2 一抗 -
描述
小鼠单克隆抗体[OTI1H2] to SENP2 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey
预测可用于: Orangutan
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免疫原
Recombinant fragment corresponding to Human SENP2 aa 139-523. Produced in E.coli (NP_067640).
Sequence:RDQPRRVLPSFGFTLNSEGCNRRPGGRRHSKGNPESSLMWKPQEQAVTEM ISEESGKGLRRPHCTVEEGVQKEEREKYRKLLERLKESGHGNSVCPVTSN YHSSQRSQMDTLKTKGWGEEQNHGVKTTQFVPKQYRLVETRGPLCSLRSE KRCSKGKITDTETMVGIRFENESRRGYQLEPDLSEEVSARLRLGSGSNGL LRRKVSIIETKEKNCSGKERDRRTDDLLELTEDMEKEISNALGHGPQDEI LSSAFKLRITRGDIQTLKNYHWLNDEVINFYMNLLVERNKKQGYPALHVF STFFYPKLKSGGYQAVKRWTKGVNLFEQEIILVPIHRKVHWSLVVIDLRK KCLKYLDSMGQKGHRICEILLQYLQDESKTKRNSD
Database link: Q9HC62 -
阳性对照
- WB: HEK-293T cells transfected with pCMV6-ENTRY SENP2 cDNA. IHC-P: Human prostate carcinoma, prostate, endometrium adenocarcinoma, pancreas carcinoma, pancreas, ovary adenocarcinoma, lung carcinoma and kidney tissues. Flow Cyt (Intra): HeLa and Jurkat cells. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SENP2.
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常规说明
Clone OTI1H2 (formerly 1H2).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI1H2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131637于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
|
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| WB |
1/2000. Predicted molecular weight: 68 kDa.
|
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| IHC-P |
1/150.
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| ICC/IF |
1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. |
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WB
1/2000. Predicted molecular weight: 68 kDa. |
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IHC-P
1/150. |
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ICC/IF
1/100. |
靶标
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功能
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. -
序列相似性
Belongs to the peptidase C48 family. -
结构域
The N-terminus is necessary and sufficient for nuclear envelope targeting. -
翻译后修饰
Polyubiquitinated; which leads to proteasomal degradation. -
细胞定位
Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 59343 Human
- Entrez Gene: 100171874 Orangutan
- Omim: 608261 Human
- SwissProt: Q9HC62 Human
- SwissProt: Q5R7K7 Orangutan
- Unigene: 401388 Human
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别名
- Axam2 antibody
- DKFZp762A2316 antibody
- KIAA1331 antibody
see all
图片
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Western blot - Anti-SENP2 antibody [OTI1H2] (ab131637)All lanes : Anti-SENP2 antibody [OTI1H2] (ab131637) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with the pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SENP2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 68 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146403-anti-senp2-antibody-oti1h2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody [OTI1H2] (ab131637)COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY SENP2 stained for SENP2 (green) using ab131637 at 1/100 dilution in ICC/IF.
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![Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146402-anti-senp2-antibody-oti1h2-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (ab131637)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SENP2 with ab131637 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
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![Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146401-anti-senp2-antibody-oti1h2-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SENP2 antibody [OTI1H2] (ab131637)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling SENP2 with ab131637 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146400-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human kidney tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146399-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human lung carcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146398-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human ovary adenocarcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146397-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human pancreas carcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146396-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human pancreas tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146395-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human endometrium adenocarcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146394-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human prostate tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)](https://www.abcam.cn/ps/products/131/ab131637/Images/ab131637-146393-anti-senp2-antibody-oti1h2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP2 antibody [OTI1H2] (ab131637)Paraffin-embedded human prostate carcinoma tissue stained for SENP2 using ab131637 at 1/50 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab131637 被引用在 2 文献中.
- Zhang X et al. Zinc deficiency induces abnormal development of the myocardium by promoting SENP5 overexpression. PLoS One 15:e0242606 (2020). PubMed: 33211757
- Bentz GL et al. Using glycyrrhizic acid to target sumoylation processes during Epstein-Barr virus latency. PLoS One 14:e0217578 (2019). PubMed: 31125383