Anti-SENP2抗体(ab58418)
Key features and details
- Rabbit polyclonal to SENP2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SENP2抗体
参阅全部 SENP2 一抗 -
描述
兔多克隆抗体to SENP2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SENP2 aa 481-530.
Database link: Q9HC62 -
阳性对照
- WB: MDA-MB-435 cell extract. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58418于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). |
|
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. -
序列相似性
Belongs to the peptidase C48 family. -
结构域
The N-terminus is necessary and sufficient for nuclear envelope targeting. -
翻译后修饰
Polyubiquitinated; which leads to proteasomal degradation. -
细胞定位
Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 59343 Human
- Omim: 608261 Human
- SwissProt: Q9HC62 Human
- Unigene: 401388 Human
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别名
- Axam2 antibody
- DKFZp762A2316 antibody
- KIAA1331 antibody
see all
图片
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Western blot - Anti-SENP2 antibody (ab58418)All lanes : Anti-SENP2 antibody (ab58418) at 1/500 dilution
Lane 1 : MDA-MB-435 cell extract
Lane 2 : MDA-MB-435 cell extract with immunizing peptide
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-SENP2 antibody (ab58418)ICC/IF image of ab58418 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58418, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab58418 被引用在 8 文献中.
- Shangguan X et al. SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis. Nat Commun 12:1812 (2021). PubMed: 33753739
- Huang D et al. Forebrain excitatory neuron-specific SENP2 knockout mouse displays hyperactivity, impaired learning and memory, and anxiolytic-like behavior. Mol Brain 13:59 (2020). PubMed: 32290845
- Zhang X et al. Zinc deficiency induces abnormal development of the myocardium by promoting SENP5 overexpression. PLoS One 15:e0242606 (2020). PubMed: 33211757
- Liu Y et al. Expression of SUMO associated proteins in the mouse endometrium is regulated by ovarian hormones throughout the estrous cycle. Exp Ther Med 19:1855-1863 (2020). IHC ; Mouse . PubMed: 32104241
- Zhang CY et al. Saikosaponin-d Inhibits the Hepatoma Cells and Enhances Chemosensitivity Through SENP5-Dependent Inhibition of Gli1 SUMOylation Under Hypoxia. Front Pharmacol 10:1039 (2019). PubMed: 31616295
- Wang H & You G The SUMO-Specific Protease Senp2 Regulates SUMOylation, Expression and Function of Human Organic Anion Transporter 3. Biochim Biophys Acta Biomembr 1861:1293-1301 (2019). PubMed: 31054272
- Wang R et al. Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2. Oncotarget 8:70142-70155 (2017). PubMed: 29050267
- Snow JW et al. Sumoylation Regulates Interaction of FOG1 with C-terminal-binding Protein (CTBP). J Biol Chem 285:28064-75 (2010). WB ; Human . PubMed: 20587419