重组Anti-SENP1抗体[EPR3844]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP1 antibody [EPR3844] (AB108981)

Immunohistochemical staining of paraffin embedded human testis with purified ab108981 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (AB108981)

Immunofluorescence staining of Jurkat cells with purified ab108981 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108981 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

• ICC/IF

AbReview29250****

Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (AB108981)

Unpurified ab108981 (1/500) staining SENP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SENP1 antibody [EPR3844] (AB108981)

Intracellular Flow Cytometry analysis of HeLa cells labelling SENP1 with purified ab108981 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• WB

Lab

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution

All lanes:

Daudi cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

• WB

Lab

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution

All lanes:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

• WB

AbReview48238****

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

Blocked with 3% milk for 1 hour at 25°C.

Incubated with the primary antibody for 18 hours at 4°C.

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution

Lane 1:

COS-1 cell lysate at 20000 Cells

Lane 2:

COS-1 cell lysate transfected with SENP1 at 20000 Cells

Lane 3:

COS-1 cell lysate transfected with SENP1 mutant at 20000 Cells

Secondary

All lanes:

HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 75 kDa

true

Exposure time: 6min

This image is courtesy of an Abreview submitted by Ragnhild Eskeland

• WB

Unknown

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 10 µg

Lane 2:

HUVEC cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

Daudi cell lysates at 10 µg

Lane 5:

U87-MG cell lysates at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa

false