Anti-SENP1抗体(ab225887)
Key features and details
- Rabbit polyclonal to SENP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SENP1抗体
参阅全部 SENP1 一抗 -
描述
兔多克隆抗体to SENP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human SENP1 aa 1-50. The exact sequence is proprietary. (NP_055369.1).
Database link: Q9P0U3 -
阳性对照
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab225887 was affinity purified using an epitope specific to SENP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 73 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity. -
组织特异性
Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine. -
序列相似性
Belongs to the peptidase C48 family. -
细胞定位
Nucleus. Cytoplasm. Shuttles between cytoplasm and nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 451862 Chimpanzee
- Entrez Gene: 102139867 Cynomolgus monkey
- Entrez Gene: 101134882 Gorilla
- Entrez Gene: 29843 Human
- Entrez Gene: 100190851 Orangutan
- Entrez Gene: 100190851 Rhesus monkey
- Omim: 612157 Human
- SwissProt: Q9P0U3 Human
see all -
别名
- SENP 1 antibody
- SENP1 antibody
- SENP1_HUMAN antibody
see all
图片
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Western blot - Anti-SENP1 antibody (ab225887)All lanes : Anti-SENP1 antibody (ab225887) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-SENP1 antibody (ab225887)SENP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225887 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225887 at 0.4 µg/ml.
Lane 1: ab225887 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab225887 被引用在 1 文献中.
- Zhu S et al. Knockdown of SENP1 inhibits HIF-1α SUMOylation and suppresses oncogenic CCNE1 in Wilms tumor. Mol Ther Oncolytics 23:355-366 (2021). PubMed: 34820505