Anti-Semaphorin 7a抗体(ab23578)
Key features and details
- Rabbit polyclonal to Semaphorin 7a
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Semaphorin 7a抗体
参阅全部 Semaphorin 7a 一抗 -
描述
兔多克隆抗体to Semaphorin 7a -
宿主
Rabbit -
特异性
ab23578 detects Semaphorin 7a protein at 75 kDa by WB. Semaphorin 7a is a protein that is tethered to the cell membrane via a GPI-anchor and is also glycosylated, therefore it is not surprising that in WB we see some additional bands at ~50 and 100 kDa which are likely to be cleavage fragments and glycosylated protein respectively. Preincubation with the immunizing peptide ab30844 is successful in blocking antibody staining. -
经测试应用
适用于: WB, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat
预测可用于: Cow
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Semaphorin 7a.
参阅Abcam的专有抗源政策 (Peptide available as ab30844.) -
阳性对照
- WB: Mouse and rat brain tissue lysate. IP: Mouse brain tissue lysate.
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常规说明
Negative control: HEK cellsThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 75, 90 kDa (predicted molecular weight: 75 kDa).
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75, 90 kDa (predicted molecular weight: 75 kDa). |
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IP
Use at an assay dependent concentration. |
应用说明
Is unsuitable for ICC/IF.
靶标
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功能
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
组织特异性
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
序列相似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
细胞定位
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 20361 Mouse
- Entrez Gene: 315711 Rat
- SwissProt: Q9QUR8 Mouse
- Unigene: 335187 Mouse
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别名
- CD 108 antibody
- CD108 antibody
- CD108 antigen antibody
see all
图片
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Western blot - Anti-Semaphorin 7a antibody (ab23578)All lanes : Anti-Semaphorin 7a antibody (ab23578) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 75 kDa
Observed band size: 75 kDa
Additional bands at: 90 kDa (possible glycosylated form)
Exposure time: 8 minutesGel type: MOPS
Blocking buffer: 2% BSA block
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Immunoprecipitation - Anti-Semaphorin 7a antibody (ab23578)Semaphorin 7a was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Semaphorin 7a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23578.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 75kDa: Semaphorin 7a.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab23578 被引用在 24 文献中.
- Su Y et al. Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development. Neuron 111:190-201.e8 (2023). PubMed: 36384142
- Li D et al. Impaired cell-cell communication and axon guidance because of pulmonary hypoperfusion during postnatal alveolar development. Respir Res 24:12 (2023). PubMed: 36631871
- Emery A et al. Dynamic regulation of semaphorin 7A and adhesion receptors in ovarian follicle remodeling and ovulation. Front Cell Dev Biol 11:1261038 (2023). PubMed: 37941899
- Körner A et al. Semaphorin 7A is protective during inflammatory peritonitis through integrin receptor signaling. Front Immunol 14:1251026 (2023). PubMed: 38094294
- Vilallongue N et al. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system. Nat Commun 13:6040 (2022). PubMed: 36229455