Anti-Semaphorin 3A抗体- N-terminal (ab226793)
Key features and details
- Rabbit polyclonal to Semaphorin 3A - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Semaphorin 3A抗体- N-terminal
参阅全部 Semaphorin 3A 一抗 -
描述
兔多克隆抗体to Semaphorin 3A - N-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow
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免疫原
Recombinant fragment within Human Semaphorin 3A (N terminal). The exact sequence is proprietary.
Database link: Q14563 -
阳性对照
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysates. IHC-P: Mouse brain tissue; rat duodenum tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226793于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
|
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| WB |
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
|
| 说明 |
|---|
|
IHC-P
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa. |
靶标
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功能
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. -
疾病相关
Hypogonadotropic hypogonadism 16 with or without anosmia -
序列相似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
结构域
Strong binding to neuropilin is mediated by the carboxy third of the protein. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 10371 Human
- Entrez Gene: 20346 Mouse
- Entrez Gene: 29751 Rat
- Omim: 603961 Human
- SwissProt: Q14563 Human
- SwissProt: O08665 Mouse
- SwissProt: Q63548 Rat
- Unigene: 252451 Human
see all -
别名
- Coll 1 antibody
- Coll1 antibody
- Collapsin 1 antibody
see all
图片
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Western blot - Anti-Semaphorin 3A antibody - N-terminal (ab226793)All lanes : Anti-Semaphorin 3A antibody - N-terminal (ab226793) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 (human neuroblastoma cell line) whole cell lysate
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 89 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody - N-terminal (ab226793)Paraffin-embedded mouse brain tissue stained for Semaphorin 3A with ab226793 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3A antibody - N-terminal (ab226793)Paraffin-embedded rat duodenum tissue stained for Semaphorin 3A with ab226793 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226793 尚未被引用在任何文献中。