Anti-SELS抗体(ab223721)
Key features and details
- Rabbit polyclonal to SELS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SELS抗体
参阅全部 SELS 一抗 -
描述
兔多克隆抗体to SELS -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Cow, Pig
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免疫原
Recombinant fragment corresponding to Human SELS aa 50 to the C-terminus.
Database link: Q9BQE4 -
阳性对照
- IHC-P: Human small intestine and pancreas tissue. WB: HEK-293 ICC/IF: U-251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223721于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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相关性
Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination. -
细胞定位
Endoplasmic reticulum; endoplasmic reticulum membrane; single pass membrane protein. -
数据库链接
- Entrez Gene: 512276 Cow
- Entrez Gene: 55829 Human
- Entrez Gene: 100151836 Pig
- Omim: 607918 Human
- SwissProt: Q2KI76 Cow
- SwissProt: Q9BQE4 Human
- SwissProt: F1SR90 Pig
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别名
- AD-015 antibody
- ADO15 antibody
- MGC104346 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELS antibody (ab223721)
Paraffin embedded human pancreas tissue stained for SELS with ab223721 (1/200 dilution) in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELS antibody (ab223721)Paraffin embedded human small intestine tissue stained for SELS with ab223721 (1/200 dilution) in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SELS antibody (ab223721)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SELS (green) using ab223721 (4 µg/ml) in ICC/IF.
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Western blot - Anti-SELS antibody (ab223721)Anti-SELS antibody (ab223721) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 21 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223721 尚未被引用在任何文献中。