Anti-SELK抗体(ab121276)
Key features and details
- Rabbit polyclonal to SELK
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SELK抗体
参阅全部 SELK 一抗 -
描述
兔多克隆抗体to SELK -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICCmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: KRRSYGNSSD SRYDDGRGPP GNPPRRMGRI NHLRGPS, corresponding to amino acids 48-84 of Human SELK.
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阳性对照
- ICC/IF: U-251 MG cells. WB: HepG2 cell lysate. IHC: Human tonsil and salivary gland tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121276于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| ICC |
Use a concentration of 0.25 µg/ml.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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ICC
Use a concentration of 0.25 µg/ml. |
靶标
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序列相似性
Belongs to the selenoprotein K family. - Information by UniProt
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数据库链接
- Entrez Gene: 58515 Human
- Omim: 607916 Human
- SwissProt: Q9Y6D0 Human
- Unigene: 58471 Human
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别名
- HSPC030 antibody
- HSPC297 antibody
- Selenoprotein K antibody
see all
图片
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Immunocytochemistry - Anti-SELK antibody (ab121276)
Immunofluorescent staining of U-251 MG (Human brain glioma) cells labeling SELK with ab121276 at 0.25 ug/ml (green).
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Western blot - Anti-SELK antibody (ab121276)Anti-SELK antibody (ab121276) at 0.04 µg/ml + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Performed under reducing conditions.Western blot analysis of HepG2 cells labeling SELK with ab121276 at 0.04 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (ab121276)ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human liver tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (ab121276)ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human testis tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (ab121276)ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELK antibody (ab121276)ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121276 被引用在 1 文献中.
- You M et al. Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis. Redox Biol 57:102500 (2022). PubMed: 36252341