Anti-SEL1L抗体(ab78298)
Key features and details
- Rabbit polyclonal to SEL1L
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SEL1L抗体 -
描述
兔多克隆抗体to SEL1L -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Hamster -
免疫原
Synthetic peptide corresponding to Human SEL1L aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab86659) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78298于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).
SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753). |
说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa). SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753). |
靶标
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功能
May play a role in Notch signaling (By similarity). May be involved in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. -
组织特异性
Highly expressed in pancreas. -
序列相似性
Belongs to the sel-1 family.
Contains 1 fibronectin type-II domain.
Contains 11 Sel1-like repeats. -
翻译后修饰
N-glycosylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6400 Human
- Entrez Gene: 20338 Mouse
- Entrez Gene: 116488 Rat
- Entrez Gene: 314352 Rat
- Omim: 602329 Human
- SwissProt: Q12916 Human
- SwissProt: Q9UBV2 Human
- SwissProt: Q9Z2G6 Mouse
see all -
别名
- IBD2 antibody
- PRO1063 antibody
- Protein sel-1 homolog 1 antibody
see all
图片
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Anti-SEL1L antibody (ab78298) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab78298 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab78298 被引用在 28 文献中.
- Weis D et al. Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death. J Clin Invest 134:N/A (2024). PubMed: 37943617
- Ji Y et al. SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool. Nat Cell Biol 25:726-739 (2023). PubMed: 37142791
- Wang L et al. HRD1 reduction promotes cholesterol-induced vascular smooth muscle cell phenotypic change via endoplasmic reticulum stress. Mol Cell Biochem N/A:N/A (2023). PubMed: 38145449
- Wu SA et al. The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes. Nat Commun 14:3132 (2023). PubMed: 37253728
- Thepsuwan P et al. Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin. Proc Natl Acad Sci U S A 120:e2212644120 (2023). PubMed: 36595688