Anti-Sec24C抗体(ab122633)
Key features and details
- Rabbit polyclonal to Sec24C
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Sec24C抗体
参阅全部 Sec24C 一抗 -
描述
兔多克隆抗体to Sec24C -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: RLPSMTGPLL PGQSFGGPSV SQPNHVSSPP QALPPGTQMT GPLGPLPPMH SPQQPGYQPQ QNGSFGPARG PQSNYGGPYP AAPTF, corresponding to internal sequence amino acids 222-306 of Human Sec24C.
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阳性对照
- U 251 MG cells; RT 4, U 251 MG, Human liver and tonsil lysates; Human stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122633于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the SEC23/SEC24 family. SEC24 subfamily. -
细胞定位
Cytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9632 Human
- Omim: 607185 Human
- SwissProt: P53992 Human
- Unigene: 81964 Human
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别名
- Protein transport protein Sec24C antibody
- SC 24C antibody
- SC24 C antibody
see all
图片
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Western blot - Anti-Sec24C antibody (ab122633)All lanes : Anti-Sec24C antibody (ab122633) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec24C antibody (ab122633)ab122633 at 1/200 dilution staining Sec24C in paraffin-embedded Human stomach tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-Sec24C antibody (ab122633)ab122633 at 4 µg/ml staining SEC24C in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab122633 被引用在 6 文献中.
- Nalbach K et al. Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2. Nat Commun 14:870 (2023). PubMed: 36797266
- Hu S et al. Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle. Nat Commun 14:7782 (2023). PubMed: 38012130
- Wei G et al. Prion-like low complexity regions enable avid virus-host interactions during HIV-1 infection. Nat Commun 13:5879 (2022). PubMed: 36202818
- Tang X et al. Export of polybasic motif-containing secretory proteins BMP8A and SFRP1 from the endoplasmic reticulum is regulated by surfeit locus protein 4. J Biol Chem 298:102687 (2022). PubMed: 36370847
- Rebensburg SV et al. Sec24C is an HIV-1 host dependency factor crucial for virus replication. Nat Microbiol 6:435-444 (2021). PubMed: 33649557
- Wang B et al. The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest 128:3319-3332 (2018). PubMed: 29939162