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AB181076

重组Anti-SEC22B抗体[EPR12335]

Anti-SEC22B antibody [EPR12335]

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(5 Publications)

Rabbit Recombinant Monoclonal SEC22B antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

查看别名

SEC22L1, SEC22B, Vesicle-trafficking protein SEC22b, ER-Golgi SNARE of 24 kDa, SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, ERS-24, ERS24

11 Images
Flow Cytometry (Intracellular) - Anti-SEC22B antibody [EPR12335] (AB181076)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SEC22B antibody [EPR12335] (AB181076)

Intracellular flow cytometric analysis ofSH-SY5Y cells (2% paraformaldehyde-fixed)labeling SEC22Bwith unpurifiedab181076 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SEC22B antibody [EPR12335] (AB181076)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SEC22B antibody [EPR12335] (AB181076)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (human neuroblastoma epithelial cell) cells labeling SEC22B with ab181076 at 1/50dilution (10.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Anti-SEC22B antibody [EPR12335] (AB181076)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SEC22B antibody [EPR12335] (AB181076)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling SEC22B with purified ab181076 at 1/50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-SEC22B antibody [EPR12335] (AB181076)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SEC22B antibody [EPR12335] (AB181076)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SEC22B with purified ab181076 at 1/250 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (AB181076)
  • IP

Unknown

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (AB181076)

ab181076 (purified ) at 1/30 dilution (2ug) immunoprecipitating SEC22B in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab181076 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181076 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (ab181076)

Predicted band size: 25 kDa,29 kDa

Observed band size: 32 kDa

false

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (AB181076)
  • IP

Supplier Data

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (AB181076)

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using unpurified ab181076 at 1/30 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (ab181076)

Predicted band size: 25 kDa

false

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (AB181076)
  • IP

Supplier Data

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (AB181076)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181076). ab181076 at 1/1000 dilution (2ug in 0.35mg lysates) immunoprecipitating SEC22B in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate. Lane 1 (input) : SH-SY5Y whole cell lysate 10ug Lane 2 (+) : ab181076 & SH-SY5Y whole cell lysate Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181076 in SH-SY5Y whole cell lysate For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution as a secondary antibody. Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (ab181076) at 1/1000 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 25 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)
  • WB

Lab

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)

All lanes:

Western blot - Anti-SEC22B antibody [EPR12335] (ab181076) at 1/5000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 28 kDa

false

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)
  • WB

Lab

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)

All lanes:

Western blot - Anti-SEC22B antibody [EPR12335] (ab181076) at 1/1000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 28 kDa

false

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)
  • WB

Lab

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)

All lanes:

Western blot - Anti-SEC22B antibody [EPR12335] (ab181076) at 1/2000 dilution

Lane 1:

Mouse brain lysates at 20 µg

Lane 2:

Mouse spleen lysates at 20 µg

Lane 3:

Rat spleen lysates

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 28 kDa

false

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)
  • WB

Supplier Data

Western blot - Anti-SEC22B antibody [EPR12335] (AB181076)

All lanes:

Western blot - Anti-SEC22B antibody [EPR12335] (ab181076) at 1/2000 dilution

All lanes:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12335

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.</p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.</p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SEC22B also known as SEC22 homolog B is a membrane protein involved in vesicular trafficking within cells. It plays an important role in the transport between the endoplasmic reticulum (ER) and the Golgi apparatus. SEC22B belongs to the SNARE (soluble NSF attachment protein receptor) family with a molecular mass of approximately 29 kDa. It is expressed in various tissues with prominent presence in the immune system and neuronal tissues. The protein participates in the assembly of vesicle transport machinery important for proper cellular function and compartmentalization.
Biological function summary

SEC22B contributes to the fusion of transport vesicles with target membranes. It acts as part of a SNARE complex facilitating vesicle docking and subsequent membrane fusion through protein-protein interaction. In immune cells SEC22B is essential for antigen presentation. It regulates the trafficking of peptide-MHC complexes to the cell surface essential for adaptive immune response. In neurons the protein ensures efficient neurotransmitter release by managing vesicle cycling and synaptic vesicle fusion.

Pathways

SEC22B integrates into the vesicular transport and secretory pathways. It operates in the ER-Golgi transport route critical in maintaining cellular homeostasis and protein processing. The function of SEC22B involves interaction with other SNARE proteins like VAMP7 and SNAP23 forming SNARE complexes that drive membrane fusion events. This protein also influences the Golgi-to-ER retrograde transport contributing to the protein sorting pathway that recycles specific proteins back to the ER.

SEC22B links closely to immune-related conditions and neurodegenerative diseases. Disruption in SEC22B function can impact antigen presentation and immune system efficiency potentially contributing to autoimmune disorders. In neurological contexts altered SEC22B activity may interfere with neurotransmitter release possibly playing a role in conditions such as Alzheimer's disease. Connections with proteins like VAMP7 underline the importance of SEC22B in maintaining cellular integrity across different systems.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
See full target information SEC22B
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 28:101225 PubMed39309162

2024

Nanoscale ZnO doping in prosthetic polymers mitigate wear particle-induced inflammation and osteolysis through inhibiting macrophage secretory autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuocheng Lyu,Xiangchao Meng,Fei Hu,Yuezhou Wu,Yurun Ding,Teng Long,Xinhua Qu,You Wang

Molecules (Basel, Switzerland) 28: PubMed37241771

2023

Extended-Synaptotagmin 1 Enhances Liver Cancer Progression Mediated by the Unconventional Secretion of Cytosolic Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Kohji Yamada,Yoshito Hannya,Tsunekazu Oikawa,Ayano Yoshida,Kuniko Katagiri,Saishu Yoshida,Rei Koizumi,Naoko Tago,Yuya Shimoyama,Akira Kawamura,Yuta Mochimaru,Ken Eto,Kiyotsugu Yoshida

Proceedings of the National Academy of Sciences of the United States of America 119:e2202730119 PubMed36044553

2022

Extended-synaptotagmin 1 engages in unconventional protein secretion mediated via SEC22B vesicle pathway in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kohji Yamada,Saya Motohashi,Tsunekazu Oikawa,Naoko Tago,Rei Koizumi,Masaya Ono,Toshiaki Tachibana,Ayano Yoshida,Saishu Yoshida,Masayuki Shimoda,Masahiro Oka,Yoshihiro Yoneda,Kiyotsugu Yoshida

Nature communications 12:4643 PubMed34330919

2021

Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Martinelli,Elmira A Anderzhanova,Thomas Bajaj,Svenja Wiechmann,Frederik Dethloff,Katja Weckmann,Daniel E Heinz,Tim Ebert,Jakob Hartmann,Thomas M Geiger,Michael Döngi,Kathrin Hafner,Max L Pöhlmann,Lee Jollans,Alexandra Philipsen,Susanne V Schmidt,Ulrike Schmidt,Giuseppina Maccarrone,Valentin Stein,Felix Hausch,Christoph W Turck,Mathias V Schmidt,Anne-Kathrin Gellner,Bernhard Kuster,Nils C Gassen

The EMBO journal 36:42-60 PubMed27932448

2016

Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Kimura,Jingyue Jia,Suresh Kumar,Seong Won Choi,Yuexi Gu,Michal Mudd,Nicolas Dupont,Shanya Jiang,Ryan Peters,Farzin Farzam,Ashish Jain,Keith A Lidke,Christopher M Adams,Terje Johansen,Vojo Deretic
View all publications
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