重组Anti-SDHB抗体[EPR13042(B)] (ab178423)
![Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.cn/ps/products/178/ab178423/Images/ab178423-343308-Ab178423KOWBDOC.jpg "Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13042(B)] to SDHB
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SDHB抗体[EPR13042(B)]
参阅全部 SDHB 一抗 -
描述
兔单克隆抗体[EPR13042(B)] to SDHB -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal liver, A431, HepG2 and Jurkat lysates; HepG2 cells; Human heart and kidney tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13042(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178423于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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| IP |
1/10 - 1/100.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
|
IP
1/10 - 1/100. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
通路
Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1. -
疾病相关
Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. -
序列相似性
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 4Fe-4S ferredoxin-type domain. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6390 Human
- Entrez Gene: 67680 Mouse
- Entrez Gene: 298596 Rat
- Omim: 185470 Human
- SwissProt: P21912 Human
- SwissProt: Q9CQA3 Mouse
- SwissProt: P21913 Rat
- Unigene: 465924 Human
see all -
别名
- CWS2 antibody
- DHSB_HUMAN antibody
- FLJ92337 antibody
see all
图片
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Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : SDHB knockout HEK-293 whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDaLanes 1 - 3: Merged signal (red and green). Green - ab178423 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab178423 was shown to recognize SDHB in wild-type HEK 293 cells as signal was lost at the expected MW in SDHB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab178423 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.cn/ps/products/178/ab178423/Images/ab178423-206387-anti-sdhb-antibody-epr13042b-western-blot.jpg)
Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.cn/ps/products/178/ab178423/Images/ab178423-206386-anti-sdhb-antibody-epr13042b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SDHB with ab178423 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.cn/ps/products/178/ab178423/Images/ab178423-206385-anti-sdhb-antibody-epr13042b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SDHB with ab178423 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.cn/ps/products/178/ab178423/Images/ab178423-206384-anti-sdhb-antibody-epr13042b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling SDHB with ab178423 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
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![Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.cn/ps/products/178/ab178423/Images/ab178423-206383-anti-sdhb-antibody-epr13042b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)Western blot analysis on immunoprecipitation pellet from (1) A431 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178423 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
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![Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.cn/ps/products/178/ab178423/Images/ab178423-7-benefits-of-recombinant-antibodies.png)
Anti-SDHB antibody [EPR13042(B)] (ab178423)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab178423 被引用在 18 文献中.
- Yang X et al. Targeting cuproptosis by zinc pyrithione in triple-negative breast cancer. iScience 26:108218 (2023). PubMed: 37953954
- Zhang W et al. Neuroprotective effect of a medium-chain triglyceride ketogenic diet on MPTP-induced Parkinson's disease mice: a combination of transcriptomics and metabolomics in the substantia nigra and fecal microbiome. Cell Death Discov 9:251 (2023). PubMed: 37460539
- Zhao H et al. Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth. Cell Biosci 13:87 (2023). PubMed: 37179385
- Wu CC et al. Reciprocal Regulation of Peroxisome Biogenesis and Myogenic Factors Is Critical for Myogenesis. Int J Mol Sci 24:N/A (2023). PubMed: 37569637
- Antony R et al. UCHL1 Regulates Lipid and Perilipin 2 Level in Skeletal Muscle. Front Physiol 13:855193 (2022). PubMed: 35464088