AB248864

重组Anti-SDHA抗体[EPR9042(B)] - BSA and Azide free

Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal SDHA antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free (AB248864)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free (AB248864)

This data was developed using ab139181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab139181 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free (AB248864)

This data was developed using ab139181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling SDHA with ab139181 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free (AB248864)

This data was developed using ab139181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling SDHA with ab139181 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free (AB248864)

This data was developed using ab139181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab139181 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free (AB248864)

This data was developed using ab139181, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SDHA antibody [EPR9042(B)] (<a href='/products/primary-antibodies/sdha-antibody-epr9042b-ab139181'>ab139181</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-SDHA antibody [EPR9042(B)] - BSA and Azide free (AB248864)

This data was developed using ab139181, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab139181 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab139181 was shown to recognize SDHA in wild-type HEK-293 cells as signal was lost at the expected MW in SDHA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab139181 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4Â°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^Â® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^Â® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SDHA antibody [EPR9042(B)] (<a href='/products/primary-antibodies/sdha-antibody-epr9042b-ab139181'>ab139181</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

SDHA knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human SDHA knockout HEK-293 cell line (<a href='/products/cell-lines/human-sdha-knockout-hek-293-cell-line-ab261853'>ab261853</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 72 kDa

Observed band size: 72 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-SDHA antibody [EPR9042(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9042(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab248864 is the carrier-free version of ab139181.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Succinate dehydrogenase complex flavoprotein subunit A (SDHA) also known as complex II Fp or SDH2 plays an important role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. It functions as a flavoprotein oxidoreductase catalyzing the oxidation of succinate to fumarate. With a molecular mass of approximately 72 kDa SDHA is expressed in the inner mitochondrial membrane of eukaryotic cells where it is a core component of the succinate dehydrogenase complex (SDHC). The complex is essential for cellular respiration and energy production.

Biological function summary

SDHA participates in the TCA cycle by accepting electrons from succinate which it donates to the coenzyme Q in the electron transport chain. This essential role connects SDHA to the regulation of ATP production in cells. SDHA operates as part of the larger succinate dehydrogenase (SDH) complex which includes other subunits such as SDHB SDHC and SDHD. This structurally integrated multisubunit complex influences mitochondrial integrity and cellular energy homeostasis.

Pathways

SDHA is deeply involved in the TCA cycle and oxidative phosphorylation pathway. As a part of these pathways it links to other critical enzymes such as fumarase and aconitase working in concert to drive the conversion of biochemical fuel into usable cellular energy. Its interactions with coenzyme Q and cytochrome complex enzymes are important for electron flow and proton gradient formation across the mitochondrial membrane. Such interactions are central to cellular respiration and energy generation.

Mutations in SDHA correlate with various mitochondrial diseases and cancer syndromes. Specifically SDHA mutations have an association with Leigh syndrome and certain types of mitochondrial complex II deficiency. These mutations disrupt the function of the SDH complex causing metabolic imbalances and energy production issues. Furthermore the integral interaction of SDHA with other SDH subunits means that alterations can impact this entire enzymatic complex with implications for cellular respiration and disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 10746566, PubMed : 24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed : 20484225).

See full target information SDHA

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi

View all publications

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重组Anti-SDHA抗体[EPR9042(B)] - BSA and Azide free