重组Anti-SDF1抗体[EPR1216] - BSA and Azide free (ab271917)
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)](https://www.abcam.cn/ps/products/271/ab271917/Images/ab271917-490978-anti-sdf1-antibody-epr1216-immunocytochemistry-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1216] to SDF1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SDF1抗体[EPR1216] - BSA and Azide free
参阅全部 SDF1 一抗 -
描述
兔单克隆抗体[EPR1216] to SDF1 - BSA and Azide free -
宿主
Rabbit -
特异性
Mouse cross reactivity based on sequence analysis only. 100% sequence homology for isforms 1-6 (P48061; isoforms 1-6).
This antibody is not suitable for endogenous detection in Western blot application.
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经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (Intra): Jurkat cells. ICC/IF: Human PBMC cells, Jurkat cells and THP-1 cells.
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常规说明
ab271917 is the carrier-free version of ab155090.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1216 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271917于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. -
组织特异性
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
发展阶段
Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney. -
翻译后修饰
Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6387 Human
- Entrez Gene: 20315 Mouse
- Omim: 600835 Human
- SwissProt: P48061 Human
- SwissProt: P40224 Mouse
- Unigene: 522891 Human
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别名
- 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
- AI174028 antibody
- C-X-C motif chemokine 12 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (Human primary peripheral blood mononuclear cell) cells labelling SDF1 with primary antibody anti-SDF1 (ab155090) at 1/100 dilution, followed by Alexa Fluor® 488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution. Anti-alpha Tubulin antibody (DM1A) - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue). Confocal image showing cytoplasmic staining in Human PBMC. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Low expression control: K562 (PMID: 23473997)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090).
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![Flow Cytometry (Intracellular) - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)](https://www.abcam.cn/ps/products/271/ab271917/Images/ab271917-2-ab155090269686antisdf1antibodyepr1216flowcytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling SDF1 with purified ab155090 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090).
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![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)](https://www.abcam.cn/ps/products/271/ab271917/Images/ab271917-3-ab155090273188antisdf1antibodyepr1216immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling SDF1 with Purified ab155090 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090). -
![Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)](https://www.abcam.cn/ps/products/271/ab271917/Images/ab271917-1-ab155090161978antisdf1antibodyepr1216immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917) -
![Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)](https://www.abcam.cn/ps/products/271/ab271917/Images/ab271917-4-benefits-of-recombinant-antibodies.png)
Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271917 尚未被引用在任何文献中。
