Anti-SCRT1抗体(ab126331)
Key features and details
- Rabbit polyclonal to SCRT1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SCRT1抗体 -
描述
兔多克隆抗体to SCRT1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
antigen sequence (LHDKGYLSDY VGPSSVYDGD AEAALLKGPS PEPMYAAAVR GELGP), corresponding to N terminal amino acids from Human SCRT1.
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阳性对照
- IHC-P: Mouse retina and brain, Human cerebellum and cerebral cortex tissue; ICC: SH-SY5Y whole cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
SCRT1 is a member of the Snail family of C2H2-type zinc finger transcription factors. It is a neural-specific transcriptional repressor that binds to E-box motifs. The protein may promote neural differention, downstream of proneural bHLH proteins, and may be involved in cancers with neuroendocrine features. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 83482 Human
- Entrez Gene: 170729 Mouse
- Omim: 605858 Human
- SwissProt: Q52LS7 Human
- SwissProt: Q9BWW7 Human
- SwissProt: Q99M85 Mouse
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别名
- hScrt antibody
- Scratch 1 antibody
- Scratch homolog 1 zinc finger protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (ab126331)
Immunohistochemical analysis of mouse retina tissue labeling SCRT1 with ab126331 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (ab126331)
Immunohistochemical analysis of human cerebellum tissue labeling SCRT1 in the nucleus of cells in the molecular layer with ab126331 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cells labeling SCRT1 in the nuclear bodies with ab126331 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (ab126331)
Immunohistochemical analysis of human cerebral cortex tissue labeling SCRT1 in the nucleus of neurons with ab126331 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (ab126331)
Immunohistochemical analysis of mouse brain tissue labeling SCRT1 in the nucleus of neurons with ab126331 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (ab126331)
Immunohistochemical analysis of human skeletal muscle tissue labeling SCRT1 with ab126331 at a 1/20 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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